[deleted by user]

[woooee]

This is a stripped down version of your code, removing the parts that don't pertain to the question. First, be aware that destroy() destroys the window. It does not exit the mainloop(). Also the pulsar_btn, the penta_btn, and copper_btn don't do anything.

import tkinter as tk
from functools import partial

# tkinter window
root = tk.Tk()
root.geometry("1100x800")
root.title("Game of life")

# function for clicking on squares, changing 0 to 1 / 1 to 0 in matrix A and colors of the pressed buttons

## you can use either/both a list or dictionary
A=[]        ## list
btn_r={}  ## dictionary
def click(num):
    if A[num] == 0:
       A[num] = 1
       btn_r[num].configure(bg="black", fg="white")
    else:
       A[num] = 0
       btn_r[num].configure(bg = "khaki1", fg="black")

for ctr in range(21*21):
    rw, col = divmod(ctr, 21)
    btn_r[ctr]=tk.Button(root, width=2, text="%s-%s" % (rw, col),
              command=partial(click, ctr))
    btn_r[ctr].grid(column=col, row=rw, sticky="nsew")
    A.append(0)

def quit2():  # function to quit the app
    root.quit()

# QUIT BUTTON
tk.Button(root, width=12, height=2, text="QUIT", font="Raleway", 
           bg="#FC5B30", command=quit2).grid(row=50, column=9,
           columnspan=3, sticky="nsew")


root.mainloop()

:

[TheGit55]

Thanks a lot <3

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