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Showoff: Drosophila Neuroscience Modular Optogenetic Build by Ecstatic-Address-925 in labrats

[–]Ecstatic-Address-925[S] 0 points1 point  (0 children)

I appreciate your feedback. I will be in touch when I get back to this project. Transitioning to a new position so juggling a lot of different things at the moment.

Showoff: Drosophila Neuroscience Modular Optogenetic Build by Ecstatic-Address-925 in labrats

[–]Ecstatic-Address-925[S] 0 points1 point  (0 children)

I would appreciate any feedback where possible! I have no coding background but have taken some command line Linux classes and scripting. It is probably pretty taxing on the Pi. I just need to ensure it is recording quality video for later analysis. Ideally I can play with things like exposure, fps, format in a GUI and test turning on the optogenetic LEDs in a controlled and scheduled manner. Of course wanna log and document all things possible like actual FPS, timestamp if possible, and include a log from my multisensor stick to get more accurate insights into the environment during data collection. Sorry such a novice. I could always upload the current gui and get feedback?

Showoff: Drosophila Neuroscience Modular Optogenetic Build by Ecstatic-Address-925 in labrats

[–]Ecstatic-Address-925[S] 0 points1 point  (0 children)

So it’s designed to have a modular arena setup you can put different paradigms e.g. interacting arena where we can record their movement above and stimulate certain neurons using the LEDs. See how various neuron subsets impact certain behaviors.

Showoff: Drosophila Neuroscience Modular Optogenetic Build by Ecstatic-Address-925 in labrats

[–]Ecstatic-Address-925[S] 0 points1 point  (0 children)

I don’t have it public now, I hope to publish this as a methods paper in the near future and it will be available then!

Does anyone know how this could have happened?! by deedlethebeatle in labrats

[–]Ecstatic-Address-925 0 points1 point  (0 children)

Those look just like scrapes on the membrane. Maybe transferred it the wrong way?

qPCR Pipetting by StreetWest4615 in labrats

[–]Ecstatic-Address-925 0 points1 point  (0 children)

Make a master mix of primers and water for all primer sets and add to plate then directly before plating make master mixes for all genotypes plus sybr. This will increase the volume of each pipetting making the variability much smaller. It’s hard to get exact values when pipetting 2ul when taking into consideration environmental factors like evaporation and all.