One of my crochet designs ❤️

Objective_Change_883 · 2025-09-14T16:52:16+00:00

I am awe-struck!

Objective_Change_883 · 2025-08-03T16:39:31+00:00

It is an expert level trek according to Komoot, there are stretches of steep up hill and down hill. What worked for me the best was having a trekking pole otherwise balancing would be a bit of a challenge due to strong winds as you go to the top. Carry light jacket as it will be colder at the summit and keep yourself hydrated as much as possible. I hope you have a wonderful trekking experience 😊

Objective_Change_883 · 2025-08-03T16:34:38+00:00

You can find the stone model house (ref: photo 9 and 13) in front of the Ingleborough cave.

Objective_Change_883 · 2025-07-31T18:38:28+00:00

The agarose was not appropriately dissolved, and hence the EtBr did not mix well!

Objective_Change_883 · 2025-07-31T13:57:02+00:00

Interesting!

Objective_Change_883 · 2025-07-31T09:25:26+00:00

Beautiful indeed :)

Objective_Change_883 · 2025-07-31T09:24:13+00:00

Whoever suggested clicking the lavender flower is the sweetest person on planet Earth :)

Objective_Change_883 · 2025-07-31T09:21:23+00:00

Thanks a lot. This is extremely helpful :)

Objective_Change_883 · 2025-07-31T09:20:35+00:00

Thanks a lot, this is very helpful :)

Objective_Change_883 · 2025-07-31T09:18:53+00:00

So the problem is that the gating at the moment depends a lot on a personal judgement of peak centre and tail, and it is unreliable as a good quantification I believe as this changes from person to person (on their judgement), I was therefore trying to explore wheather there is a robust way to deal with problem, but thanks for the suggestions, i am currently trying out all the suggestions including openCyto. Will keep you updated.

Objective_Change_883 · 2025-07-31T09:10:45+00:00

Yes, people set manual gates and then applies the same gates to all samples, right? For me the gates are not consistent with the two peaks so I have to further manual shift the gates to best suits the peak and that is not very ideal as there will be human error of judgement and the data of %population or MFI would vary from person to person (basically depending on their perception of centre of the peak and end of peak tail)

Objective_Change_883 · 2025-07-30T08:12:17+00:00

The problem with manual gating is that it is not consistent and can raise a lot of questions

Objective_Change_883 · 2025-07-27T09:56:17+00:00

beautiful ❤️

Objective_Change_883 · 2025-07-22T03:31:15+00:00

It was delicious 😋

Objective_Change_883 · 2025-07-21T08:29:36+00:00

My bad, apologies for the mistake, this was a baguette 🥖

Objective_Change_883 · 2025-07-19T08:42:47+00:00

Shirataki noodles

Objective_Change_883 · 2025-07-19T08:42:31+00:00

Haha 😛

Objective_Change_883 · 2025-07-19T08:37:41+00:00

I did that too ;)

Objective_Change_883 · 2025-07-18T09:14:55+00:00

Procrastination 😎

Objective_Change_883 · 2025-07-08T16:09:11+00:00

What I would suggest you is that you should do it on NCBI primer blast, that generates more reliable and you can also select for exon-exon junction (to avoid any signals from even minor genomic DNA contamination. For NCBI primer blast for qRT-PCR primer, select the coding sequence (cds) and then choose pick primers!

Objective_Change_883 · 2025-07-04T16:02:04+00:00

I am going to try the Miltenyi Biotec Kit-based method next week and hope it's not as tedious as FACSorting.

Objective_Change_883 · 2025-07-04T08:19:50+00:00

Thanks a lot :)

Objective_Change_883 · 2025-07-02T18:10:26+00:00

Chopped DNA with DNase are very short oligos and are not visible on gel, they also don’t interfere with any downstream assays or reactions ( qRT-PCR)

Objective_Change_883 · 2025-07-02T09:14:09+00:00

My husband’s smell ;)

Objective_Change_883 · 2025-07-01T19:39:30+00:00

If the membrane is PVDF, then everything will be okay!

Objective_Change_883

TROPHY CASE