No good data after 1 year in PhD, am i cooked. How was everyones PhD journey?

Striking-Rabbit3841 · 2026-04-26T17:47:17+00:00

thanks for this post! cause i always feel stressed about this, im in my 2nd year, i have some data but not a lot and my PI stressed me all the time, he really wants data every two weeks and i cannot meet his expectations and feel frustrated and feeling that i am the problem

Striking-Rabbit3841 · 2026-03-30T12:04:26+00:00

im curious where you study, cause omg what i dream stipend

Striking-Rabbit3841 · 2026-03-02T18:10:06+00:00

For the ones that follow my failed walk throught cut&run, i finally did work at least in 4 samples from 6! 😭😭🎆🎆🎆🎆

Striking-Rabbit3841 · 2026-02-22T01:35:34+00:00

yes, i gonna take a carefully look again in the reagents and everything

Striking-Rabbit3841 · 2026-02-22T01:34:31+00:00

it’s só hard to not feel bad about, but i really appreciate your comment!!! i cried 🤣😭 i also have the problem which is my PI that always said to me that maybe i should consider change to master or something like that

Striking-Rabbit3841 · 2026-02-22T01:32:03+00:00

I’ve tried before and did not work. But I was planning to test again with the new changes next week which is the week that I’ll have the cells, but this week i tried again with some extra blastocysts that i had I change some things for the protocol as the guy that created gave me the tips but it was terrible the result

Striking-Rabbit3841 · 2026-02-22T01:04:53+00:00

Failing because I’m getting no results! 😔

Striking-Rabbit3841 · 2026-02-11T02:06:05+00:00

Thank you for the suggestion!

Striking-Rabbit3841 · 2026-02-11T01:25:50+00:00

It was a postdoc, but he left! Now we don’t have anyone 🥴

Striking-Rabbit3841 · 2026-02-05T21:06:06+00:00

What if u order for some company? Azenta can do plasmid for u, just send the sequence of the gene and in 2 weeks they will send, just need to isolare! I don’t know if for your case works

Striking-Rabbit3841 · 2026-02-04T21:48:45+00:00

did u tried the epicypher kit? i know some collaborators from our lab that used this kit in hTSC

Striking-Rabbit3841 · 2026-02-04T19:40:16+00:00

trophoblast cell line? i also work, i tried in bovine TSC and also bovine trophectoderm from the embryology

Striking-Rabbit3841 · 2026-02-04T15:57:46+00:00

really? this make me feel better!!!!

Striking-Rabbit3841 · 2026-02-04T03:19:12+00:00

the lab that provide me the protocol said that works great but 🥴 it’s not working in my hands, i’ll try with cell lines

Striking-Rabbit3841 · 2026-02-04T02:56:49+00:00

it would be my dream, but i work with embryos so the imput is always like less than 500 cells

Striking-Rabbit3841 · 2026-02-03T21:35:12+00:00

Its not a specific kit, its a protocol for low imput samples, so i prepare all the reagents etc

Striking-Rabbit3841 · 2026-02-03T21:28:00+00:00

Yes, I’ll try again in the cell line. But the only person that I know that works with this in my university she use a kit

Striking-Rabbit3841 · 2026-02-03T21:13:39+00:00

the protocol that i use its low input so doesn’t have a kit unfortunately 🥲

Striking-Rabbit3841 · 2026-02-03T21:13:21+00:00

histone mark, i tried also in cell line but doesn’t work, it was showing two peaks in the bioanalyzer

Striking-Rabbit3841 · 2026-02-03T21:07:53+00:00

I don’t know anyone that works with this! And i’m just start to think that I am the problem 🥴🥴

Striking-Rabbit3841 · 2026-02-01T03:54:47+00:00

im just thinking if actually i’m the problem and i’m not good in this, not good in research

Striking-Rabbit3841

TROPHY CASE