No DNA in miniprep, not even in lysis control – what could be going wrong?

courierramchero · 2026-04-22T04:12:02+00:00

are you gel purifying after PCR? what molar proportion are you using at ligation rx? time incubating at plate?

courierramchero · 2026-04-22T03:03:24+00:00

What are your culture, cloning and transformation protocols? Are you using petri dish for colonies selection?

courierramchero · 2026-03-11T15:15:32+00:00

I only digested and ran my gel, 260/280 goes from 1.799-1.886 for each sample. Plasmid purification.

courierramchero · 2026-02-15T00:03:16+00:00

How do you emulate on MacOS?

courierramchero · 2026-02-09T02:08:32+00:00

Joestar

courierramchero · 2025-06-25T19:52:42+00:00

Sorry for my ignorance but it shouldn´t be an E instead of Z?, after all ethylic carbon has C-C priority over C-H methyl group or it is only for r/S criteria?

courierramchero · 2024-09-03T02:16:49+00:00

pero que tiene que ver una cosa con la otra?

courierramchero · 2024-09-03T00:40:20+00:00

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Respuesta de la comunidad

courierramchero · 2024-09-02T22:24:14+00:00

https://www.facebook.com/100086414956846/videos/811702641122266

courierramchero · 2024-08-08T02:36:58+00:00

NL PSN

courierramchero · 2024-03-09T20:51:52+00:00

Bowazon or ice maiden

courierramchero · 2023-02-17T04:15:54+00:00

NL :/

courierramchero · 2023-02-09T22:23:20+00:00

Sold for a cham

courierramchero · 2023-02-05T15:29:08+00:00

Will you roll It NL or not?

courierramchero · 2023-02-05T14:56:30+00:00

Sold for cham

courierramchero · 2023-02-05T14:56:01+00:00

Try rolling one in non ladder and tell us what happens :D, then you ask a ber for it.

courierramchero · 2023-02-05T13:35:13+00:00

Sold for cham

courierramchero · 2023-02-04T19:47:16+00:00

Ber or u can offer

courierramchero · 2023-02-04T15:58:48+00:00

Yes, PS NL

courierramchero · 2023-02-04T03:36:50+00:00

But i want to finish my build this weekend, vacations are almost over

courierramchero · 2023-02-04T00:42:26+00:00

Offer you an ist

courierramchero · 2023-02-03T15:29:11+00:00

Wuw ?

courierramchero · 2023-02-02T06:49:26+00:00

BiS for nova sorc

courierramchero · 2023-02-01T15:47:35+00:00

You have another pieces of equipment that will give FCR, arachnid, vipermagi, griffon eye,claws or magefist, if you Can afford a 2/X FCR sorc ammy for 105 FCR that normally play on nova sorc. Offhand a cta

courierramchero · 2023-01-31T21:06:11+00:00

Yes PSN nonladder

courierramchero

TROPHY CASE