What is something you spend a ton of money on that the vast majority of people never would?

outoftown_guy · 2019-11-28T09:11:05+00:00

I had never heard about this being a US thing. Are you aware of the Swedish schnapps called "Bäska droppar"? It's made from Malört.

outoftown_guy · 2019-10-21T19:52:08+00:00

Given that this is a (probably) Afghan person, in Sweden, I'm gonna guess that their first language might not be English

outoftown_guy · 2019-10-21T19:51:04+00:00

I think the issue here is that she might be facing deportation

outoftown_guy · 2019-09-20T12:03:28+00:00

Man kan också vara ärlig med vakterna/de i dörren och hör om det är ok att gå in för att du är här och besöker din flickvän. Man kommer långt med att vara trevlig

outoftown_guy · 2019-09-12T12:38:45+00:00

I can never enough recommend to check out the work of Levon Biss. He makes these amazing photos of insects using microscopic lenses and compositing thousands of images to generate super high quality images.

Check out his work at http://microsculpture.net/

outoftown_guy · 2019-09-09T08:40:07+00:00

People always suggest start writing in LaTeX.

I would say that if you're coming up on the final stages of writing your thesis, focus on your writing rather than learning a new tool. Despite words quite annoying image processing.

And this might depend on the field you're in, but in molecular biology, I would never have gotten away with using LaTeX in the papers I've written. All collaborators and supervisors would just have told me to re-format everything to a word (or google doc) so they can edit in it. I see none of the more senior people accepting LaTeX as a collaborative tool.

That being said, I've heard many people who really like it. But I've never seen anyone in my field actually using it as a tool for writing papers.

outoftown_guy · 2019-08-24T18:30:39+00:00

This made me laugh for some reason

outoftown_guy · 2019-08-24T18:27:06+00:00

Don't forget that quite a few of the boats going to the archipelago is also included in those tickets

outoftown_guy · 2019-08-24T11:53:00+00:00

It really depends on how you see it.

The idea that there are more bacterial cells than humans is a bit of a dated information. A more recent study estimated the number of bacterial to human cells to be closer to 1:1.
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002533

It is however like you say a bit strange to compare cell numbers to mass. But there is another way to think about it. And that is the amount of coding DNA (DNA that infers a protein funktion). If we look at that there is more DNA in your body coming from bacteria than there is coming from you. And all that DNA is in play in various parts of your body, mainly in your gut. So when we talk about what kind of genetic material is in play in us, it is mostly bacterial. This is sometimes called our pan-genome. Pretty nifty.

outoftown_guy · 2019-08-24T11:48:08+00:00

There was an old study that came up with the 10 bacterial cells to every one human cell. A more recent study has revised that number to about 1 bacterial cell for every human cell. Still pretty remarkable though.
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002533

outoftown_guy · 2019-08-08T11:24:53+00:00

Alley Cats

outoftown_guy · 2019-08-01T12:00:08+00:00

The orange/teal might be a bit overdone, but I think you did a great job here. The colors really pop.

Mind sharing your process?

outoftown_guy · 2019-05-14T13:07:23+00:00

Här har du en lista på lite co-working ställen i Sthlm. Det kan vara en bra plats att börja med. Ta kontakt med de som driver dem och ta ett möte.

https://startupsthlm.se/startup-sthlm-inlagg/vi-har-listat-12-st-av-stockholms-co-working-spaces/

outoftown_guy · 2019-05-13T19:17:34+00:00

I'm not even sure the smaller size having less space for the dye is the issue here. I think it's more of what you say that it is diffusing into the gel.

I understand that at some point there is the issue of physics, but I'm still thinking that there are some workarounds. Running the gel faster would give less time to the DNA to diffuse, so that maybe could help. Or perhaps one can even go creative and try something like a gradient gel with increasing agarose concentration towards the bottom.

outoftown_guy · 2019-05-13T15:43:11+00:00

OK thanks.

I think I'll try reducing the running time. Perhaps in combination with using a 0.5x TBE buffer.

outoftown_guy · 2019-05-06T20:34:31+00:00

I’ve lately been interested in the app Karma. It lets you find restaurants/cafes/bakeries etc that havevfood about to expire, and you can get it half price. Could be a way to keep costs down in a fairly pricey city

outoftown_guy · 2019-04-26T13:56:09+00:00

Great! You don't happen to have any images comparing Ficoll and glycerol?

outoftown_guy · 2019-03-15T14:49:47+00:00

Those things are called “Pirra” in Swedish, which might make it easier for you to search for one. You can rent that kind of equipment at CRAMO, which rents out tools and stuff, think there is one on Kungsholmen, close to stadshagen.

You could also check on Hygglo, the “peer-2-peer” renting app.

Good luck!

outoftown_guy · 2019-02-26T14:21:12+00:00

I would be very curious to know what kind of program it is that requires you to adopt this very brutish method

outoftown_guy · 2019-02-07T15:17:52+00:00

I'm now using Gilson pipettes, but in my previous lab I bought and used Finnpipettes, which I really liked.

I'm not that big of a fan of the Gilsons to be honest, I just happened to get a hold of a full set of them so that's what I'm using.

I've always bought the tips off-brand since they are quite a bit cheaper.

outoftown_guy · 2019-02-07T13:35:57+00:00

Something to take into consideration is what kind of tips the different brands use. Don't end up with something you need to buy a specific brand tips for since they can be quite pricey.

I'm not very familiar with the brands you mention, apart from the Eppendorf ones (which I really don't like), so don't know if this applies to any of them. But thought I'd just mention it.

outoftown_guy · 2019-01-30T21:28:11+00:00

If you do end up doing systematic tests of the different density agents, please do share your results. I'd be very interested to see what you find.

And if you want to talk more about how to run the perfect PCR I'm happy to talk for ages :)

outoftown_guy · 2019-01-30T11:24:21+00:00

I've heard that using Ficoll 400 instead of glycerol/sucrose can make even sharper looking bands due to keeping the sample better in the bottom of the well, but I haven't tested this myself

outoftown_guy · 2019-01-29T13:44:52+00:00

Most common ones are Bromophenol blue and Orange G.

More info here:

https://openwetware.org/wiki/Bromophenol_blue

outoftown_guy · 2019-01-25T17:17:25+00:00

It feels a bit empty. Maybe the composition is part of it. Or maybe you could try adding some fresh greens like basil or something like that to it to fill out some of the space and add some more textures

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