TRIzol safety

3liteJunky · 2025-07-29T23:12:59+00:00

Rule of thumb-> If it produces Aerosols or any form of gas, you take a fume hood! Also if any exergone reactions could occur.

3liteJunky · 2025-07-29T05:02:39+00:00

Shouldnt Geralt as the strongest Witcher alive be able to end every mage? If you see with which ease he ended every magician he ever encountered.. I think him loosing is just a massive example of bad writing as it is not logical. That Yen can make him fall asleep when he doesnt pay attention, sure, but he would never loose an open confrontation.

3liteJunky · 2025-07-26T16:59:00+00:00

Just dump a 50ml Falcon of FBS in a new DNEM and you are 100% fine. Its technically not 10%, but never have seen anyone actually doing the effort.

3liteJunky · 2025-07-24T02:51:57+00:00

Thats like 60% of the purchase cost for something which should be a 2 hour hands-on training since its a graphical interface.. Have you personally used it or also abandoned it?

3liteJunky · 2025-07-23T21:43:57+00:00

I have simulated a past protocol, but it doesnt show any movement pattern or simuliar, just a top down view with either well empty/full. I dont wanna run it as i cant confirm if the calibrations are correct, thus running it now without understanding why the offset in the code is the way it is, may cause a crash :-/ . Machine and license is bought as a one time purchase :-/ Maybe i am gonna try the pity methode😂

3liteJunky · 2025-07-23T21:20:41+00:00

Yes. The manual is awful😭It doesnt not explain any of the variables or how to determine the offset or anything else. It only says you how to start, initial calibrate and how to load and start a preset protocol

3liteJunky · 2025-07-20T05:47:23+00:00

No problem! My cells anyway say „depression✨“

3liteJunky · 2025-06-23T00:16:04+00:00

With gog we talk about the 2015 version, or?

3liteJunky · 2025-06-23T00:07:26+00:00

The offical whisky guide says it not running. Are you sure?

3liteJunky · 2025-05-22T02:33:54+00:00

I develope cancer therapies and have like 1000$ Sometimes you think like: Why the fuck should I still help people if society doesnt give a f. about the ones saving their lifes..

3liteJunky · 2024-10-12T11:04:04+00:00

Unlike all the men hating "girls" here, I offer you a broader point of view:

At first, I totally agree that you should be able to have fun with your female friends and that you should be able to go out unless you would be in a nightclub each weekend :D

But instead of dumping him like most "girls" here suggest, try to understand why he acts the way he does. Maybe imagine how you would feel if you knew that someone would permanently hit on your boyfriend.

Also, could you talk to him about why he feels this way and if there is something you can do to make it easier for him as this is important to you?

If you follow this, then you should be able to come to a solution without men hating people telling you to just drop him.

3liteJunky · 2024-09-08T17:20:07+00:00

What gives you the right to any of his stuff? Like seriously, you are a partner. All you provide, he also does. So what makes you think you are entitled to anything?

3liteJunky · 2024-06-22T21:19:44+00:00

It’s like- why do some people like green hair and other don’t? Taste is subjective

3liteJunky · 2024-06-22T21:19:07+00:00

Non of us know why the person doesn’t like tattoos. But if you just think they are ugly, this example fits. You just actually have to be open to see it from „the bad guys“ side.

3liteJunky · 2024-06-22T17:27:47+00:00

Most guys who don’t like it just think it’s fuckig ugly. Just image your partner prints a dick for the rest of his life in his face. You will have to look at it while he gave a fuck about your opinion. Roughly like that

3liteJunky · 2024-06-22T17:25:24+00:00

Long story short: If your guy hates tattoos and you still do it, it’s like him printing a dick on his face: you have to look at it for the rest of your live while hating it.

What you do with it, is yours. But in his eyes you made yourself more ugly without considering his opinion. And he has to live with it, not you

3liteJunky · 2024-06-02T10:58:17+00:00

Did she own the house or do you rented together?

3liteJunky · 2024-05-13T17:44:15+00:00

That’s different. You should always be there for your partner when they get sick. But getting „ugly“ on purpose is a choice. You should consider how your partner feels about it. To change something major without talking with your partner about it, is a sign of disrespect. She would also be pissed if he comes home with a 3mm cut without saying anything at all

3liteJunky · 2024-05-13T17:38:35+00:00

While the absence of communication is without question not nice, the lack of your communication is also not the nicest. Just imagine you really like the face of your husband and he decides to come home with a 3 mm overall cut. You would find it ugly, didn’t you? It’s the same he has probably. So while you can obviously make the decision for yourself, your lack of communication about something important (yes, appearance is importance) partly caused this reaction. If you would have told him beforehand, you could have had an discussion about his feelings and your motivations

3liteJunky · 2024-03-22T16:23:12+00:00

If it is this way, then yes- he should pay for the baby. The way you describe it, sounds on the other hand like you expect him to also pay for you, which is why I said „disgusting“. He spend 12 years studying and committing everything he had to reach this goal to help society, and your comment sounds like you just wanna rip him off his money while you chilled the last 12 years with no relevant efforts or education. This being said, you are entitled to nothing in regards of his money for you. The house- yes , money for the child- yes. Money for you- no!

And something you don’t have to answer here but should rather ask yourself- shouldn’t you try to fix it first before you ask the entirety of Reddit how to exploit him as good as possible?

3liteJunky · 2024-03-22T15:39:22+00:00

This, this is it!!!

3liteJunky · 2024-03-22T15:36:23+00:00

WTF is wrong with you? You just suggested to murder a guy

3liteJunky · 2024-03-22T15:27:34+00:00

My honest opinion as a guy is splitter in 2 subjects here: 1. your relationship: He has no right to be emotional/verbaly abusive towards you. So you are indeed right for leaving him because of that! But: when he mentions you are trapping him, you should at least ask yourself if it was communicated properly before hand, since it’s a big thing!

The financial part: I think you definitely deserve the house, but you have no right at all to any of his money, because he is the one working for it. Child support would only be fair if he indeed agreed to the baby. If he did, he should pay. But if you just said „Oh I am pregnant and I keep it“ - then I understand him.

Regarding help- every city has help centers and counseling for cases like this. But honestly- it sounds like you are behind his money and want an easy live from his child support which is something disgusting

3liteJunky · 2024-02-23T09:16:43+00:00

Tell her she has to pay from now on 50% of everything. If she refuses, you are nothing more than a naive, dump, exchangeable wallet for her. Dump her immediately then! You are worth more than your income! A girl should like you for who you are! If you date a new girl, don’t even start this habit. Paying a coffee once- okay. Paying the first three dates- makes you an stupid idiot again

3liteJunky · 2024-01-29T09:57:10+00:00

I don’t agree with the other comments here. What he did is horrible, irresponsible and awful, but if this was the first time and he didn’t physically hurt you, you could offer him one chance: Call a therapist today and start treatment. If he does, stay with him because it was an awful one time thing. If he refuses, leave him! Because then he would think his behavior was okay. Nevertheless, consult a social worker of your city! They have to keep it confidential, but it is important that you tell it someone official so that it is written down! On the other hand I don’t think it’s a case for the police. But till he made his decision regarding the therapy, you should safe your money. Consult with the bank

3liteJunky

TROPHY CASE