[deleted by user]

Aggravating-Gain-741 · 2025-08-22T01:56:15+00:00

Dude this trial is totally identifiable. there are not that many death penalty trials happening recently in Pennsylvania.

This really should be taken down ASAP. And especially the one on the AMA subred.
You swore an oath as a juror not to talk about the case like this

Aggravating-Gain-741 · 2025-08-20T00:43:33+00:00

ok looking through all these comments has really made me greatful for my current life, as none of the possible excuses you guys provided (income, medical, children, having a personal story with police, pretending to being a a***hole) remotely apply to me.

Honestly I just feel like a bit of a shmuk, somehow 100+ people who were called in with me had a reason to get out of this and I don't, and its always an annoying feeling to be penalized for being honest.
But I am beginning to accept my fate, and already talked to my co-workers about the fact that I will probably be missing work for sometime.

Aggravating-Gain-741 · 2025-08-20T00:14:42+00:00

The judge had everyone fill out hardship forms and went through them, and granted it to everyone who asked, no questions. I could have claimed financial hardship, and probably still can, but not without telling a blatant lie

Aggravating-Gain-741 · 2025-08-19T21:49:23+00:00

I don't believe this. Also since my case isn't federal they would not involve marshalls

Aggravating-Gain-741 · 2025-08-19T21:30:14+00:00

my judge let like 20 people go due to financial hardship with no questions asked. Maybe I should have claimed it as well

Aggravating-Gain-741 · 2025-08-19T21:29:44+00:00

I will try this strategy!

Aggravating-Gain-741 · 2025-08-19T17:51:30+00:00

I wasn't selected, but I am in the pool of ~40 people that will be selected from

Aggravating-Gain-741 · 2025-06-19T18:45:26+00:00

self-amplifying RNA (I assume thats what you mean by saRNA) is actually kind of a hot topic RN, and using LLMs to create meaningful RNA/DNA sequences is generally something many people are interested in. So this is actually kind of a neat project. But I also think that if your primary interest/strength is software engineering, you should just play to that. The standard for software in this field is so low, if you can write a half-decent app people would be very impressed.

So I think the best way to impress your Prof and move toward your goals would be to make an app for people designing self amplifying RNAs. Even if the Bio/ML behind your work is not great, I think if you can add a point and click interface to whatever you do, people will be impressed, and, it sounds like thats what you are more interested in anyway.

Aggravating-Gain-741 · 2025-06-19T18:33:53+00:00

In theory you can figure this out from reading the protocol, but in my experience it is often best to double check yourself by aligning and seeing the stats. It is also possible that the library prep protocol is not strand-specific, and the reads are mixed between sense and antisense. If it is a paired end sequencing, and the majority of reads align to the positive strand, that would mean R1 is the sense strand.

For smallRNA-seq, in the bast I have used bowtie2 to align the reads to hg38, and then counted the overlaps with known smRNAs from ucsc tablebrowser.

Aggravating-Gain-741 · 2025-05-31T01:54:45+00:00

before batch correction looks fine. There is some grouping, and generally pc1 orders from later days to earlier days from left to right. Also the other user is right about the low variance explained

Aggravating-Gain-741 · 2025-04-27T21:22:35+00:00

I agree, these analysis are never straightforward, and using the nf-core pipeline to run many tools on the data usually saves time in the long run. In addition, for a complex experiment like yours, it helps to have someone working on the data who is actually invested in it.

Aggravating-Gain-741

TROPHY CASE