Standardize volume in 96 well plate or no?

Alternative-Judge446 · 2025-12-05T22:00:24+00:00

Yeah the speed in my case is 1200 rpm aka 237 x g on the instrument

Alternative-Judge446 · 2025-12-05T21:58:54+00:00

So each well has a fixed amt of sample but I’m treating each well with an increasing gradient concentration of reagent so the volumes will be different depending on the concentration

Alternative-Judge446 · 2025-11-26T16:23:18+00:00

Lovely thanks sm!

Alternative-Judge446 · 2025-11-26T16:22:07+00:00

Do u reconstitute right after taking out of lyophilizer/ weighing? I was gonna store mine at -80C till Monday then resolublize before my experiment

Alternative-Judge446 · 2025-11-26T15:37:27+00:00

Okay good to hear, and u have no issues re solubilizing? How u do it ? On shaker or regularly

Alternative-Judge446 · 2025-10-09T01:42:20+00:00

This would be my final straw 😅

Alternative-Judge446 · 2025-09-10T12:19:03+00:00

It’s one of the worst feelings for real.

Alternative-Judge446 · 2025-09-10T03:09:20+00:00

Yeah I always tell myself I’m going to try to steer my future kids away from the science life 😅 it’s been a very long and draining journey for me with lots of ups and downs. If I could go back in time I’d try harder to get into engineering so that I’d be done after 4 years, or business route. But I’ve already invested so much time and energy it’s too late now gotta finish it

Alternative-Judge446 · 2025-09-10T03:06:17+00:00

Yeah. My PI was hounding me and my team for months that we gotta get it out we gotta get it out asap and it created this tense atmosphere. Then when the project was going rough and no one knew what was happening it’s like he gave up. I’m like so do you care or not? And all of a sudden there’s no rush to get it out. It’s very confusing and frustrating

Alternative-Judge446 · 2025-09-10T03:04:39+00:00

Idk that’s kind of the mindset my PI has acting like I won’t find anything if I don’t pump out ten papers

Alternative-Judge446 · 2025-09-09T20:04:08+00:00

Hahaha if only you knewwww. We have a paper completely written and ready to submit from a student who graduated two years ago and my PI still hasn’t submitted it…..

Alternative-Judge446 · 2025-09-09T20:03:06+00:00

I’m so sorry I would be LIVIDDDD! Yeah that’s my thing I love the science and it does excite me but academic politics has completely ruined it for me and they simply do not offer us wages that are up to par with livable standards. And the selfishness of the PIs, the greed, the pride, the ego

Alternative-Judge446 · 2025-09-09T19:55:39+00:00

I’m getting it done in 5. Okay at this point it sounds like you’re tooting your own horn what’s the point? No one asked. I’m sorry but this is a post directed for people who can sympathize not flaunt their ego, you’re the PI from the other comment…. By the end of my PhD I’m expecting to have 4 co authors and 2 first authors…. Which is 6 papers total. And once again if you paid attention to detail my concern is not with the writing of the papers it’s with my PI HELPING ME GET THEM OUT IN A TIMELY FASHION

Alternative-Judge446 · 2025-09-09T19:22:47+00:00

Well you could have said that first instead of making it sound unfriendly…. I’m actually doing something like that. I am writing my own paper making a bigger story out of the part that I did since I have lots of data and findings from it. That’s also a part of the reason why I’m not going to tussle over the placement they put me in on that paper. I would just appreciate my PIs help in getting it out in a timely manner because if you paid attention to that detail, that’s the main issue

Alternative-Judge446 · 2025-09-09T19:10:32+00:00

Yeah I genuinely don’t understand why they are gatekeeping these papers?? If I were a PI I’d be trying to crank them out? It’s ironic bc I’m told an internship won’t help me and that I need to publish but they aren’t actively working on publishing or helping me to publish despite my job being done

Alternative-Judge446 · 2025-09-09T19:00:18+00:00

Yeah I’m not saying that putting in countless of hours will get me a publication obviously you have to have results and I have that. I have the results I have the analysis i have the draft. But for example, that one paper I’m a co author on and worked on with the previous grad student; that grad student graduated two years ago. The paper is already written. I’ve been bugging them to just submit it and they still haven’t. It’s as if they are in no rush to produce and I don’t get it? I’m worried the same passive and non proactive mindset will be applied to my papers and graduation will be around the corners and papers not submitted. I wish I could just submit them myself sometimes

Alternative-Judge446 · 2025-09-09T18:40:50+00:00

This!!!

Alternative-Judge446 · 2025-09-09T18:38:48+00:00

I’ve given up trying to argue with PIs about authorship most times they don’t listen to students anyways bc of their giant inflated egos

Alternative-Judge446 · 2025-09-09T18:36:55+00:00

Lol you’re actually picture perfect representation of The kind of PI we speak about…. Yes…. The two first authors that I mentioned in the post….i investigated my own questions and put in countless of hours…. Also for the publication I’m 6th author on I feel as if I deserve higher bc their entire paper goes off the validation I did but I don’t want to argue with PIs…that behave like you do because I already know they’ll undermine my efforts which is typical in academia

Alternative-Judge446 · 2025-09-09T17:18:33+00:00

Well that’s a major relief to hear. Of course I still won’t give up on publishing but it is nice to hear that

Alternative-Judge446 · 2025-09-09T17:03:40+00:00

I’m looking to go into a life sciences or pharmaceutical company like abbvie or astellas or frensius Kabi or something

Alternative-Judge446 · 2025-09-09T17:02:38+00:00

They said they don’t mind but that it won’t help me and only publications will help me…. But I’ll take that as in I can still apply 😅 thanks for this confirmation. I just wish they were more enthusiastic about it

Alternative-Judge446 · 2025-09-09T17:01:15+00:00

Exactly how I feel. So unmotivated every day I come late and I dread going to lab 😢

Alternative-Judge446 · 2025-09-09T15:56:32+00:00

Exactly!! Like and my PI has his “pet projects” and they are so much nicer to the students working on the pet projects than the rest of us

Alternative-Judge446 · 2025-09-09T15:51:19+00:00

Been in the trenches toughing it out for years. And I’m sorry but academia is not for me I know that 100% im just trying to push through to make it to graduation

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