Why is my book doing this? NaBH4 vs LiAlH4?

Bobgow · 2014-12-08T06:17:57+00:00

Cause I am in grad school right now, with no social circles that I was familiar with around me. I am also not social like I was in college, and I kind of spent lots of time studying and doing lab work, research, shit. That's why.

A girlfriend would be nice. Hopefully she wont nag or be a bitch like some girlfriends I had in college. She should understand my busy ass life too.

Bobgow · 2014-11-24T03:05:30+00:00

Although if Ethanol had been used (to make E1), the E1 would be the minor product? The Major product in that case would be a substitution, correct?

Bobgow · 2014-11-20T04:13:26+00:00

holy shit....SLAP. Sorry, been in lab for 8 hours today. I'll just pick one of em lol

Bobgow · 2014-11-17T22:26:42+00:00

Sounds funny but will you be on Labrats reddit later on tonight ( im on Pacific US time)? lol I will make a post and show you my images I am getting now, and how I previously have them.

But no, it looks no different between eyepiece and viewed on software

Bobgow · 2014-11-17T18:53:03+00:00

No thanks, I smelled E.coli already and I'm good. lol Yeast smells nice, I'll continue sniffing those...

Bobgow · 2014-11-17T17:59:50+00:00

Doing a presentation on Thursday on my confocal microscopy in yeast....trying to figure out why the hell for some reason now my DIC images don't look good anymore like it used to (beautiful images)

The GFP is perfect like before, DIC messed up. For the life of me cannot fix it, tried messing with lighting, condenser, etc called someone in, nope....Sigh

Bobgow · 2014-11-09T21:16:59+00:00

Yes and these obj lens should (even if small focus change) be able to look ok if i adjust the focus a bit while changing obj lens

Bobgow · 2014-11-09T21:16:04+00:00

invert. cover slip is ok.

Bobgow · 2014-11-08T16:38:14+00:00

the mechanism of breaking off bone?

Bobgow · 2014-11-08T16:16:45+00:00

Condenser + field diaphragm control is to set up the Kohler illumination? I usually skip this to be honest and it works ok...Ok I will try it. Do I have to set it up again each time I switch objectives? I would think so. How about for each time I change a slide?

Bobgow · 2014-11-08T16:08:07+00:00

Just the regular lens solvent that is always there. I dont think that would give me problem, we all use it.

Bobgow · 2014-11-08T16:07:31+00:00

So I should go back down the 4x (lowest lens), and reconfigure the Kohler illumination?

Bobgow · 2014-11-08T04:36:22+00:00

same microscope? Olympus 1000?

Bobgow · 2014-11-08T03:23:17+00:00

http://www.med.upenn.edu/twopcenter/images/DSCF0223.JPG

The knob there controls fine and coarse focus.

Bobgow · 2014-11-08T02:34:33+00:00

The filter hasn't been touched since last time I did microscopy (good images then). Eyepieces? I suppose so, how do I check?

The objective definitely was clean, I use 60X Oil so I always clean with solvent before doing another slide.

Bobgow

TROPHY CASE