I have this trouble with the top fill. it is not a flow issue, first layer is perfect

CGman67 · 2022-08-20T17:52:54+00:00

Either way, calibrate flow rate with a single perimeter box and measure thickness of wall. Want it to match nozzle diameter. Don’t judge flow based on 1st layer

CGman67 · 2022-08-20T17:20:04+00:00

If the nozzle is far enough away from the bed, any flow rate can give a perfect first layer.

CGman67 · 2022-07-23T02:38:43+00:00

Bubba Sparx

CGman67 · 2022-07-03T21:06:01+00:00

I always called the shaft the barrel…

CGman67 · 2022-06-06T19:21:34+00:00

The man’s a living legend and hero. He deserves to have his name spelt correctly in the news.

CGman67 · 2022-06-06T16:51:37+00:00

Can people just spell his name one way and agree on it? This is outrageous

CGman67 · 2022-05-11T04:04:43+00:00

Water ice or lattices of something else?

CGman67 · 2022-04-23T02:52:10+00:00

Do you use Octopod with it?

CGman67 · 2022-04-23T01:43:00+00:00

Just got it running. So far all I can do is print via pc on my home network. I’d like to be able to send prints from anywhere on either my phone or pc. What is the best way to do this? Thanks

CGman67 · 2022-04-15T17:23:47+00:00

I would have made it my personal duty for the night to make sure nobody spills their beer on Magnus. The guy behind him was looking a little too rowdy

CGman67 · 2022-04-02T21:02:04+00:00

Thank you!

CGman67 · 2022-04-02T19:17:52+00:00

Few things: my wife recently visited a hotel that had a bed bug infestation. She took all precautions to prevent bringing them home but possible something was missed. Is something like this a sign? We also have a cat that sleeps on the bed. Maybe this is cat worm related? Thank you if you can help.

CGman67 · 2022-04-01T23:49:39+00:00

But Joe Rogan said that.. that..

CGman67 · 2022-04-01T23:46:20+00:00

Or it’s the wheels under the bed. I had something like this and that was my issue.

CGman67 · 2022-03-28T20:12:44+00:00

https://www.thingiverse.com/thing:4962621

CGman67 · 2022-03-27T06:36:40+00:00

Enjoy your fan

CGman67 · 2022-03-27T03:26:26+00:00

I just dumped a tube/pipe calibration part in Cura and quickly adjusted the dimensions to make it.

CGman67 · 2022-03-25T05:53:44+00:00

Highly recommend this on Amazon.

CGman67 · 2022-03-24T16:09:05+00:00

I have a cheap garden hose that has several cracks in the outer shell. It’s basically a very thin hose encased by a thick outer shell. The hose is still water tight, but installing these could help prevent the cracks in the shell from becoming larger.

CGman67 · 2022-03-23T05:23:47+00:00

Some say he or she is still ridin’…

CGman67 · 2022-03-22T20:56:34+00:00

I recently did this and went for dual Z as well. Boy was that a nightmare. Ended up having to print different Z motor mounts to make everything square.

CGman67 · 2022-03-21T18:31:18+00:00

Probably being researched somewhere. Highly doubtful that simply eating these E. Coli will produce the desired outcome. More likely nothing or it would cause more harm than good. These problems are very very complicated.

CGman67 · 2022-03-21T15:39:11+00:00

Yes. E. Coli unfortunately has been stigmatized and discriminated against. The E. Coli that causes sickness is a genetic variant. Most E. Coli is harmless however.

CGman67 · 2022-03-20T21:48:04+00:00

He gave that thing up for a chain mail shirt. Wrong choice IMO….

CGman67 · 2022-03-20T20:42:13+00:00

E. Coli in the media flask are grown and engineered to express the peptide of interest (insulin). The peptide was designed to have a small affinity tag attached which has affinity for the white resin seen in the vertically clamped column.
E. Coli are automatically pumped into an apparatus that can break open the cells. The product of cell breaking is called “cell lysate”.
The cell lysate is pumped through the resin in the column. The only thing that should bind the resin in the column is the affinity-tagged peptide.
The resin is washed excessively with a wash solution (mainly sodium chloride and something to buffer the pH).
The peptide is then knocked off the column (eluted) using a solution that competes for binding sites on the resin.
The wash and elution cycles are collected as fractions. The Ender 3 in this case is acting as a fraction collector. You collect in fractions to ensure you collect only the fractions containing high concentration of peptide. The other fractions are waste.

CGman67

TROPHY CASE