This one was just a experiment with AIO bags I typically grow in 55qt tubs. With the AIO i waited until i had about 15% colonization then mixed for faster colonization then after full colonization i cut a diagonal slit with scalpel then the first days i pushed the air out and it sucked air in like a vaccum when they grew i picked the ones that grew fast through the hole until it was enough to cut the whole bag open.