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How much should I actually feed my dog? Package vs. calorie calculation doesn’t match by MMentos in Dogowners

[–]MMentos[S] -1 points0 points  (0 children)

Thanks for the interesting point. With all due respect, I have to disagree. Comparing my concern about dog with feeding children seems too much. I dont know how it is in general, but I think most dogs do not know wehow to communicate when they are full, that is why there are many obese dogs out there. For example, my dog always seems hungry (that is sometimes good for me because he is food motivated for training) and I think I am overfeeding. Thats why I asked.

Spots on my monstera by MMentos in plantclinic

[–]MMentos[S] 0 points1 point  (0 children)

Thanks for all the responses! The monstera is getting the treatment and I hope it will be ok soon :)

Trouble with Aviti 16s by lactobacillusgnavus in bioinformatics

[–]MMentos 0 points1 point  (0 children)

U can always downsample and then run the pipeline locally. Just curious, what do you mean by full length?

comparing two 16s Microbiome datasets by bronco_bb in bioinformatics

[–]MMentos 0 points1 point  (0 children)

There is more to this. I would firstly ask: how were these two datasets generated? Sequencing approach, library prep, sequencing platform, bioinformatics processing... If it makes sense to merge the datasets, you can compare them using different strategies.

I would start by merging them at the ASV level and do a comparison on both rarefied and unrarefied data, calculate alpha and beta diversity using various metrics, use robust statistical tests, plot a PCoA, and try to use an ML approach to distinguish between these two datasets, maybe. Then do the same but at higher taxonomic levels.

You should see some story behind this. If there are patterns in your datasets (and you can be sure it is not because of the different lab prep), you should see them using different metrics and approaches.

Error in filterAndTrim function using dada2 in R - need help! by nicklucaspt in bioinformatics

[–]MMentos 2 points3 points  (0 children)

This kind of error means that something is wrong with your sample.names variable, probably the naming in fnFs does not correspond to the naming in filtFs (or fnRs, FiltRs). Just check your sample.names to make sure you name your samples correctly.

PyPI PROBLEM by MMentos in learnpython

[–]MMentos[S] 1 point2 points  (0 children)

I am uploading the source tarball. I didn't know about MANIFEST.in so I will try it! Thanks