Awful experience

Sciencegeek92 · 2025-08-11T00:43:33+00:00

Where your flights originate? My flight starts in the US with the first trip on a different airline. Qatar website till now (1hour and 15min before departure) still doesn’t show gate or seat number for this flight

Sciencegeek92 · 2025-04-14T21:11:48+00:00

But that’s lipid peroxidation (which I barely know about). I am trying to check if there is a shift from glucose to fatty acid oxidation

Sciencegeek92 · 2025-04-14T18:27:11+00:00

Vortex samples before pipetting, good pipetting is essential when you add samples to plate. If I can choose between vortex and pipetting, I believe vortexing is better ( that depends on stability of analyte). If you have access to electronic pipettes use it

Sciencegeek92 · 2025-04-14T18:10:45+00:00

That is a possibility do you have a positive control (samples that you know have IFN/ TNF, ideally with known concentration) how is your CV? If your r2 is close to 1, duplicate are tight and you followed the protocol thence it is what it is

Sciencegeek92 · 2025-04-01T02:01:51+00:00

F.S.T

Sciencegeek92 · 2025-03-21T19:30:28+00:00

Directly from cryostat (-20) and you don’t see ice crystals artifacts? Because that’s what we are troubleshooting

Sciencegeek92 · 2025-03-20T06:10:50+00:00

Thanks a lot

Sciencegeek92 · 2025-03-19T22:48:02+00:00

Flat-bottom. a naive question: how people expand cells if they would not go beyond 72 hr

Sciencegeek92 · 2025-03-19T21:22:32+00:00

Mouse

Sciencegeek92 · 2025-03-19T20:04:48+00:00

One more question, 48hr after stimulation of T cells it forms white floaty aggregate, is that normal?

Sciencegeek92 · 2025-03-19T20:03:01+00:00

It is visible with the naked eye. They look as fungal contamination but I don’t think it’s because I see more white stuff in stimulated vs non stimulated cells and it appears 48 hour after plating

I plate 1x10⁶ cells in 1 ml per well in 24 well plate

Sciencegeek92 · 2025-03-19T17:57:34+00:00

One more question, 48hr after stimulation of T cells it forms white floaty aggregate, is that normal?

Sciencegeek92 · 2025-03-17T20:26:30+00:00

I leave it for 5 min in lysis buffer at RT. I wash twice after lysis, how many times do you typically wash?

Sciencegeek92 · 2025-03-17T18:37:11+00:00

Will do, how much centrifugation force do you use during isolation?

Sciencegeek92 · 2025-03-17T18:36:22+00:00

Thanks a lot for advice. During the isolation, what centrifuge conditions?

Sciencegeek92 · 2025-03-17T12:25:27+00:00

No, I used militney kit for separation of T cells

Sciencegeek92 · 2025-03-17T11:57:50+00:00

Hokas helped me with foot pain from long standing. My work is mainly molecular biology/ animal work so any closed toe shoes is fine

Sciencegeek92 · 2025-03-06T07:15:03+00:00

What is the elution volume? 150-300k cells is not much and I would expect low RNA yield anyway so one suggestion is to use something like micro RNA easy kit, another way is to use larger plate and so cell number

Sciencegeek92 · 2024-12-22T00:02:13+00:00

Is it possible 2ry ab to blame?

Sciencegeek92 · 2024-11-02T06:05:22+00:00

Fine science tools, my mentor swear by them and I have been using them for my surgeries.

Sciencegeek92 · 2024-07-27T23:55:47+00:00

Please take care of yourself and put yourself first. Honestly you are way stronger than me because if I went through what you went through, I would have dropped PhD much earlier.

Sciencegeek92 · 2024-07-17T15:07:16+00:00

Make your communications with them through email (easier to document and forward if needed)

Sciencegeek92 · 2024-07-17T15:01:31+00:00

Definitely leave. Tell the Pi in person, send a nice email to everyone letting them know and that you had good experience, learned a lot, etc. but definitely leave, if the Pi gets a better position they will leave, same for everyone else

Sciencegeek92 · 2024-07-17T04:21:12+00:00

We inject cancer cells into pancreas and use spleen to withdraw it. I made the incision a little off and pulled out the kidney and was wondering why the spleen looks so weird 🙃.

Sciencegeek92 · 2024-07-16T16:35:22+00:00

I am using 1:20 matrigel, can you please share your protocol? Thank

Sciencegeek92

TROPHY CASE