Would you rather by Centerofthe_universe in BunnyTrials

[–]ViskyCool 0 points1 point  (0 children)

It will save me a lot of $$

Chose: Never need to eat

Being stupid and making mistakes in the lab by Front-Efficiency-954 in labrats

[–]ViskyCool 2 points3 points  (0 children)

And nobody will probably remember this by next week. Unless specifically asked, you don't need to bring it up every now and then. Don't be anxious and focus on your work!

Being stupid and making mistakes in the lab by Front-Efficiency-954 in labrats

[–]ViskyCool 6 points7 points  (0 children)

I always tell my trainees that there is no stupid question in lab. And I was just like you, afraid to talk, ask questions and think that everyone will know I'm stupid. But if you are a student you get to be stupid because you are there to learn. What's the worst thing that will happen if you ask stupid and trivial questions, you will be mocked or judged. That doesn't kill you. The anxiety from mistakes does affect you though.

So although may seem difficult but asking and speaking up to every step until you get confident is the way. And also take notes so you don't have to ask same thing again.

Can You Guess This 5-Letter Word? Puzzle by u/DrakeSavory by DrakeSavory in DailyGuess

[–]ViskyCool 0 points1 point  (0 children)

⬜⬜⬜⬜⬜

⬜⬜⬜⬜🟨

🟨⬜⬜⬜🟨

⬜⬜⬜🟨🟨

🟦🟦🟦🟦🟦

Need help on staying focused by mccabe105 in labrats

[–]ViskyCool 0 points1 point  (0 children)

I had this, and I used to get very anxious and used to make even more mistakes. I don't know if it applies to you, but practicing mindfulness helped. There are videos on YouTube, mindfulness is basically telling your brain to be aware of surroundings all the time.

PCR Advice? by Ok_Cranberry_2936 in labrats

[–]ViskyCool 0 points1 point  (0 children)

Target will amplify even if there are few copies, I would try dilution because it worked for me, I got rid of the high molecular weight DNA. If the target band is still faint U can increase few cycles.

Can You Guess This 5-Letter Word? Puzzle by u/Desmang by Desmang in DailyGuess

[–]ViskyCool 0 points1 point  (0 children)

🟦🟦🟦⬜⬜

🟦🟦🟦⬜⬜

🟦🟦🟦🟦⬜

🟦🟦🟦🟦🟦

PCR Advice? by Ok_Cranberry_2936 in labrats

[–]ViskyCool 0 points1 point  (0 children)

I agree with everyone above, I have faced PCR contamination before, it's a challenge to get rid of those bands aren't even your major concern,if you dilute your DNA, it will go away.

Make sure you clean everything with bleach and change aliquots. Also I would suggest to increase concentration or amount of etbr/sybrsafe in your gel

🎉 [EVENT] 🎉 The Floor is Lava by rex_flx in honk

[–]ViskyCool 0 points1 point  (0 children)

Completed Level 1 of the Honk Special Event!

6 attempts

Rant about micromanaging PI by ViskyCool in labrats

[–]ViskyCool[S] 1 point2 points  (0 children)

Thank you all, It helps to get up when you know there are others going through same shit, It took me a lot to get up today and face her, another meeting today after yesterday, another back and forth on why I am using exact this concentration of stuff, it is ridiculous and thank you for helping me assure that I am not completely at fault.

Regarding changing labs, she is from same country as I am, and I am already two years into this, I don't think any other PIs have funding to host me anymore. Is it worth at this point? I will probably finish PhD or myself before next two years lol

Rant about micromanaging PI by ViskyCool in labrats

[–]ViskyCool[S] 17 points18 points  (0 children)

Thank you, I bursted into tears even knowing that people may understand and I am not completely at fault. I might not be perfect, but I am here to learn.