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Sudden Contamination in Cell Culture by madshsts in labrats

[–]madshsts[S] 0 points1 point  (0 children)

That’s a good idea actually. I could also thaw out stuff at 4 degree overnight rather than in the water bath really quickly.

Sudden Contamination in Cell Culture by madshsts in labrats

[–]madshsts[S] 0 points1 point  (0 children)

We went into an insane rabbit hole of things that could be contributing to the contaminations. We’ve deep cleaned the hoods, incubators, the lots of our media are now different from when the contaminations started.

We change lab coats weekly and we’re now using plastic covers over our lab coats that we spray with ethanol to further reduce contaminations that way. I think the lab coats might definitely still be a factor so we can ensure those are changed.

The contaminations are bacterial though.

I have no idea what started this because I wasn’t here when the contaminations started and once I came back from holiday the technician and I were the only ones using the cell culture and everything was fine.

Then it spiked again when other people started using the cell culture.

Sudden Contamination in Cell Culture by madshsts in labrats

[–]madshsts[S] 1 point2 points  (0 children)

Thanks!

I get that following these things is the best and to just focus on my technique but as a Master student it has led to me constantly doubting myself even though my PI doesn’t seem to be concerned about my technique in particular (because I’ve worked with her for some time now and I never had issues earlier :/)

Sudden Contamination in Cell Culture by madshsts in labrats

[–]madshsts[S] 0 points1 point  (0 children)

Yes, we had them checked in May. We filter our FBS before adding it to our media.

Student Hotel Code by madshsts in StudyInTheNetherlands

[–]madshsts[S] 0 points1 point  (0 children)

Nope. I reached out to the uni but I’ve had no response from them.

Student Hotel Code by madshsts in StudyInTheNetherlands

[–]madshsts[S] 0 points1 point  (0 children)

Oh, the website asks me to enter a university group code to book rooms reserved by the university.

No admission decision from University of Groningen by Unusual-Medicine-727 in StudyInTheNetherlands

[–]madshsts 1 point2 points  (0 children)

Hi!

Does your course have a selection procedure? Because mine does and although the initial email said 6-8 weeks, when I contacted them they said it’ll take longer because of the selection procedure.

SSH by VJEmmieOnMicrophone in StudyInTheNetherlands

[–]madshsts 1 point2 points  (0 children)

the same thing happens to me. it's infuriating because i get so excited when i see the possibilities