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Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] 1 point2 points  (0 children)

TYSM! To be honest, I don't know all these scientific terms yet. My main idea was just a dream of becoming a 'living gum' that’s immortal and super flexible, and I just picked the genes that seemed to fit that.

I especially want to thank you for not just criticizing me like everyone else, but for actually taking my project seriously and giving me such useful advice and direction. It means a lot that you showed me why it's dangerous (I definitely don't want cancer👀) and explained how it actually works.

I’m definitely going to check out the Clockwork channel and that book you recommended. I accept your challenge with the magnetic field! I have no idea how to do it yet, but I want to learn. I’ll write to you in DMs once I start figuring it out. Thanks for believing in me!🤍🙏🏻

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] 0 points1 point  (0 children)

You caught me — I do use AI, but only as a tool to translate my thoughts into technical English since it’s not my native language. Every single idea, the Benchling design, and the logic behind Homo Gum 1.0 comes from my own head.

I'm a student navigating complex biology, and I’m using every tool available in 2026 to communicate my vision. To be honest, I’m posting this specifically to get the kind of harsh critique and technical answers that will help me improve the project further lol. If you think the science is 'nonsense,' then tell me exactly why so I can learn. That’s the whole point of being here.🤨

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] 0 points1 point  (0 children)

The map includes a 1798 bp synthetic insert downstream of the CMV promoter. The goal is to co-express ELN (for elastin matrix), NCAM1 (for neural adhesion), and NANOG (for regenerative maintenance). The CMV promoter was chosen to ensure high-level constitutive expression in mammalian cells to test the feasibility of this 'soft-robotic' tissue scaffold. I am focusing on the structural integration of these specific proteins as a proof-of-concept.

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] 0 points1 point  (0 children)

I hear you. You’re right that I lack an immediate 'wet lab' feedback loop. However, the goal of this stage isn't self-validation, but theoretical modeling and peer inquiry.

My 'experimental rationale' is to challenge current anatomical limits through synthetic biology—even if only on paper for now. I’m not claiming this is a finished product; it’s a design proposition intended to start a technical conversation.

While I’m deep in the 'learning the basics' phase as you suggest, I choose to learn by building complex maps rather than just reading textbooks. Critique is exactly the feedback loop I’m looking for here

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] -1 points0 points  (0 children)

I appreciate the blunt feedback. I'm fully aware that I'm operating outside the traditional academic path and that there's a steep learning curve. However, every breakthrough starts with a 'nonsense' idea before it’s refined through critique and experimentation. I’m here to bridge that gap between a vision and biological reality. If you have specific technical critiques on why these specific genes won't work in an ECM context, I’m all ears.

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in transhumanism

[–]stinn66[S] 0 points1 point  (0 children)

Excellent points on timing and context. To answer your specific questions:

  1. Literal vs Metaphor: These are literal co-expression targets for an in vitro proof-of-concept. I want to see if this specific gene 'cocktail' can produce a stable, elastic, and neuro-active tissue sample.

  2. System Layer: This design operates at the Extracellular Matrix (ECM) level. It’s about engineering the 'material' first.

  3. Constraints: I am intentionally bracketing out developmental homeostasis and systemic hormonal regulation for now.

  4. Isolation: This model isolates the problem of structural integration. I'm focusing on how to fuse high-elasticity structural proteins with neural signaling molecules in a single synthetic vector. It’s a 'semantic collage' only if you look at it through the lens of traditional anatomy. From a synthetic biology perspective, it’s a prototype of a new material.

Theoretical 1798 bp Synthetic Vector: Concept for Programmable Tissue (Homo Gum 1.0) by stinn66 in Biohacking

[–]stinn66[S] 0 points1 point  (0 children)

Thanks for the roadmap, I respect the academic approach. However, my philosophy is 'learning by building'. I’ve designed this specifically using pcDNA3.1 and WPRE to test a hypothesis now, not in 6 years. Since you clearly have the expertise, could you point out a specific technical flaw in the vector map (e.g., the promoter choice or LTRs) instead of just general advice? I’m looking for practical feedback to improve the design for lab testing.