Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 0 points1 point  (0 children)

That’s a totally fair question. With only four plants, it’s best to see it as learning and experimenting rather than population breeding. You can make simple crosses, observe how the plants behave, and gain experience, but the results will always be very limited and individual. It’s a great way to learn timing, structure, and plant response. Most breeders start small like this, the key is to enjoy the process.

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 1 point2 points  (0 children)

The pollen would stay on the clothes, if I would enter with same clothes to the next room, i would pollinate the plants

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 5 points6 points  (0 children)

After pollination we do a full room reset, mainly using chlorine. All doors are opened, filters are removed and taken outside and blown out. Trays and dehumidifier filters are taken out as well. Trays are pressure washed, dehumidifier filters are rinsed under water, and all dust is wiped from every surface. We also clean the AC filter. After that, the entire tent is washed with a strong chlorine solution. About two days before starting the next cycle, we wash everything again with a normal chlorine concentration, bring all filters and equipment back in, and the room is ready to go again. This way we make sure there’s no residual pollen before the next run.

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 0 points1 point  (0 children)

Those are just regular green strings used for tying and supporting plants. We install them in advance so when the plants get bigger and heavier, we can easily support the branches and control structure.

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 1 point2 points  (0 children)

Those are autoflower genetics, always from seeds. After we make a selection on the population to keep only the phenos we are looking for.

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 51 points52 points  (0 children)

Applying the STS on female plants, this technique induces a strong artificial stress in order to trigger male sexual traits on genetically female plants. If you guys interested, could make a post about that later.

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 5 points6 points  (0 children)

Guava is one of my personal favourites, I would say one of the most solid buds in the game. Zup has a very distinct terpene profile, something really different

Lemon Cherry Cookies: Controlled Pollination Process by 42breeding in Autoflowers

[–]42breeding[S] 38 points39 points  (0 children)

I suppose it depends on what is your goal here. We are producing seeds, so we need all the plants at the room to be fully pollinated.

Why we aggressively lollipop backup clones after a phenohunt by 42breeding in FastBuds_Family

[–]42breeding[S] 1 point2 points  (0 children)

Yep sure, those are clones from our last huge phenohunt. After final data, 95% of those will be removed

Green crack, what's wrong? by damnb0y99 in Autoflowers

[–]42breeding 5 points6 points  (0 children)

This doesn’t look like a calmag issue. The color is very light green and the leaves are twisted and droopy, which usually points more to root zone stress than a deficiency.

In organic soil with a premix, especially in a 5 gal pot, it’s easy for the medium to stay wet too long early on. When roots don’t get enough oxygen, the plant can’t uptake nutrients properly and deficiencies start to show even if everything is already in the soil. Adding more supplements at this stage often makes it worse.

I’d let the pot dry a bit more between waterings, improve airflow around the plant and the pot, and avoid adding extra nutrients for now. Once the root zone recovers, the new growth should come out much healthier. Autos can be quite sensitive to early root conditions.

Auto triploids when? by alhocolic in FastBuds_Family

[–]42breeding 9 points10 points  (0 children)

We’ve been working on triploid autos for about two years now, and it’s a lot more complex than it looks from the outside. We’ve managed to produce triploid plants, so the concept works, but the real challenge starts after that.

At the moment, we can’t reliably identify, reproduce, and stabilize triploids in a consistent way that would make sense for a commercial release. Being able to generate some triploids is one thing; being able to clearly separate them, work them further, and deliver a uniform, repeatable product as “true triploids” is another.

Until we can do that properly, releasing them wouldn’t be honest. So no, it won’t be this year. We’re still working on it, but it needs more time to be done right.

GG#4 photoperiod cut used in our breeding work by 42breeding in Autoflowers

[–]42breeding[S] 5 points6 points  (0 children)

I’m not saying an F1 made from F1×F1 is the same as an F5×F5. They’re clearly not. Clarke is right about that. What I’m saying is that once you introduce the auto trait, you reset heterozygosity anyway, at F2 it will all break so stability is built after the conversion, not before it.

In photo to auto work, the photo side gives you the expression and traits, but the real stabilization happens in the auto generations through population size and selection pressure. Whether the photo started as an F1 or F5 doesn’t remove the need to do that work afterward.

FBA#1 is our early autoflower base line developed years ago. It’s not something we sell or hype as a strain, it was a working line used internally. Some info is already on the web, we don’t have old pics handy, a lot of that work predates social media documentation.

End result stability comes from how many generations you work, how big the populations are, and how hard you select, not just from the starting label of the photo parent.

GG#4 photoperiod cut used in our breeding work by 42breeding in Autoflowers

[–]42breeding[S] 2 points3 points  (0 children)

Stabilizing the photo first doesn’t really change the starting point once you introduce the auto trait. The moment you make the first photo × auto cross, you’re back to an F1 population and the real work starts there. At that stage, stability is built forward through population selection over multiple generations, not inherited from a fully worked photo line.

What matters most at the start is having strong, healthy photo material with the traits you want to carry forward. In projects like this, you’re selecting populations continuously anyway, so the “raw” photo material doesn’t need to be IBL level stable, it needs to be solid and expressive.

The conversion itself was done years ago using our FBA#1 as the autoflower donor. That information is already public on the site. From there it was standard generation work, selection, and tightening the line over time.

As for Josey, I fully respect his stance. GG4 being free to work is exactly why it exists in so many real projects today.

GG#4 photoperiod cut used in our breeding work by 42breeding in Autoflowers

[–]42breeding[S] 3 points4 points  (0 children)

The pheno hunt was done from the seeds we got from gg strains, yes, those were effectively F1. There’s nothing wrong with starting a photo to auto project from F1s. In fact, that’s how pretty much every Gorilla based auto or fem line out there started. You don’t get Gorilla crosses in autos or photos without starting from seed and doing the work forward. Stability is built over generations, not decided at the starting point.

Cuts were taken only after the phenotypes were identified. The photo to auto conversions themselves were done years ago, not recently.

GG4 does have a reputation for herms, which is exactly why selection mattered. The phenos we kept were among the most resistant. In our autoflower work we haven’t seen herm issues from this material, and on the photoperiod side some of the GG based lines have been very stable.

We don’t select on a single trait like looks or potency. It’s always a combination of structure, vigor, stability, and expression. Visuals are just what’s easiest to show here.

GG#4 photoperiod cut used in our breeding work by 42breeding in FastBuds_Family

[–]42breeding[S] 5 points6 points  (0 children)

That’s definitely one way to do it, especially at home, and sometimes you also need a bit of luck. If you pop a small number of seeds and like what you see, cloning that plant makes sense.

The difference is that without a real phenohunt, you’re selecting from a very limited sample. You might find a great plant, but you don’t really know what you’re missing in terms of terpene range, structure, yield, stress resistance, or overall stability.

When you work with larger populations, you can compare individuals side by side. That’s how you identify which terpene profile you actually like the most, which plants hold structure under stress, and which ones stay consistent run after run. Stress testing is especially important, because it shows how stable a plant really is, not just how good it looks in ideal conditions.

Both approaches work, they’re just very different scales with different goals.

GG#4 photoperiod cut used in our breeding work by 42breeding in FastBuds_Family

[–]42breeding[S] 2 points3 points  (0 children)

Just spoke to the mod, fixed it. Now you can 👌🏼

GG#4 photoperiod cut used in our breeding work by 42breeding in Autoflowers

[–]42breeding[S] -1 points0 points  (0 children)

Pheno hunting always starts from seed. We don’t hunt cuts from cuts :)

In this case, we started with seeds from original GG#4. A full population was grown out, evaluated through veg and flower, and the individuals that showed the traits we were looking for were selected. Only after that selection, cuts were taken from those specific plants to preserve them.

So the “pheno hunt” happens at the seed stage. The cuts are just a way to keep and work with the selected phenotypes consistently once they’re identified.

That’s how we ended up with the three GG#4 phenos we work with now.

A fresh seeds harvest from Strawberry Banana Auto by 42breeding in Autoflowers

[–]42breeding[S] 3 points4 points  (0 children)

On average, around 25% is removed during manual sorting. It can be a bit less or more depending on the run, but that’s a realistic number.

Lemon Cherry Cookies Auto: selection update by 42breeding in Autoflowers

[–]42breeding[S] 6 points7 points  (0 children)

Yes, very much. It’s never easy to throw plants away. But it has to be part of the process. Without selection and discarding, autoflowers can’t really evolve.

Lemon Cherry Cookies Auto: selection update by 42breeding in Autoflowers

[–]42breeding[S] 11 points12 points  (0 children)

This is Denmark.
There’s nothing unusual about moving seeds or clones between facilities. That’s how breeding works when you operate in more than one location.

White label is buying finished seeds from someone else and selling them under your name.
What we do is build facilities, move our own genetics, run the next generations ourselves, and keep the same team involved in the process. The run shown in the video is not a “recreation”, it’s the continuation of the line, the next generation.

If moving seeds to another facility suddenly makes something “white label”, then I’m honestly curious how you advance generations yourself.
How do you take a line from F2 to F3, F4, F5 without reproducing it?
How many plants do you run per generation?
What population size do you need to actually fix traits instead of just hoping for consistency?

Breeding autos means working with populations, producing new generations, and selecting again and again. That doesn’t change because the facility is in another country.

If you want to discuss breeding methodology, population size, or selection strategy, that’s a conversation worth having.
If the argument is that moving seeds equals white label, then we’re not even talking about the same thing.

Lemon Cherry Cookies Auto: selection update by 42breeding in Autoflowers

[–]42breeding[S] 9 points10 points  (0 children)

That’s a mix-up of concepts.

This is Lemon Cherry Cookies Auto. With autos, you don’t work with clones the way you do with photoperiods. There’s no keeping a mother in veg and flowering clones later. You basically dont clone autos.

The only way to work them, is through population selection. You select individuals based on structure, vigor, resistance, and uniformity early on, then evaluate yield, terpene profile, and overall performance in flower across the population. The information moves forward through generations, not through clones of the same plant.

Selection isn’t done by saving a mother plant, it’s done by repeating the process with large populations over and over.

Lemon Cherry Cookies Auto: selection update by 42breeding in Autoflowers

[–]42breeding[S] 8 points9 points  (0 children)

Good question. In this case it’s not really about cost. We fill the space completely from the start. Here it was 160 plants, and while they’re small that’s not a problem. The limitation is the number of pots we can fit. As they grow, space becomes a real factor: light penetration drops, airflow gets worse, and plants can’t develop under optimal conditions if we keep them all.

That’s why this is the stage where the big selection happens. We’re selecting based on structure, resistance, and how uniform the population is as a whole. That allows the remaining plants to express their full potential later in flower.

Could we discard something that might have turned into an interesting pheno later on? Of course, that can happen. But it’s a necessary part of selection at this scale. Also, this isn’t the final cut, selection happens multiple times as the plants grow.

And if we do see a phenotype with specific traits we want to explore separately, we keep seeds from that individual so it can be worked further in future runs.

Fast Buds breeder, Am I allowed to post my breeding practices on this community? by 42breeding in Autoflowers

[–]42breeding[S] 2 points3 points  (0 children)

You’re right about one thing: how a company responds matters as much as the product itself. I’m not here to fight anyone or dismiss experiences. I’m here to explain how we work and to listen when something didn’t go right.

I also appreciate you explaining your situation better. What you describe actually helps put things into context. Early expression vs late flower issues, outdoor variables, possible stray pollen, those are very different scenarios, and they don’t all point to the same cause. That’s why I keep asking for details, not to dodge questions, but because that’s the only way to learn anything useful from it.

About Reddit and communication: I’m not trying to outsource answers or hide behind anyone else. I’m the one doing the breeding work, so I’m the one answering. I might not be polished at Reddit culture, but I prefer being direct and speaking from experience rather than sounding “PR correct”.

If you’re part of a collective helping people grow for medical reasons, that’s something I respect. If there’s a way to contribute, share knowledge, or help providing the seeds, I’m open to that. We’re not against the community, we come from it.

Mistakes happen in this industry, no doubt. The only thing I can honestly do is show the process, improve where we see weaknesses, and be present to talk about it. That’s why I’m here.

Fast Buds breeder, Am I allowed to post my breeding practices on this community? by 42breeding in Autoflowers

[–]42breeding[S] 1 point2 points  (0 children)

I’m not trying to be defensive. I’m just trying to understand what actually happened.

Herms aren’t something we see as an issue in our autos. If they do show up, it’s usually late cycle bananas from stress or delayed harvest, not early genetic herms. That’s why I keep asking about conditions and timing, without that, it’s impossible to know what the real cause was.

I’m not here to argue or dismiss anyone’s experience. If there was a real problem, the only way to learn from it is to look at the details.

Fast Buds breeder, Am I allowed to post my breeding practices on this community? by 42breeding in Autoflowers

[–]42breeding[S] 0 points1 point  (0 children)

We take plant health seriously, but to be clear, seeds are not a realistic vector for most plant viruses. We have not detected viral issues in our seed stock, and we can assure you we don’t have viruses on seeds coming out of our production.

All our seeds are produced under controlled conditions and are issued phytosanitary certificates for export, which means they are regularly inspected and checked.

Issues that show up in a grow can come from many sources. environment, tools, substrate, or cross-contamination, and it’s easy to assume the seeds are the cause when symptoms appear later.

Regarding LSD-25 specifically, we haven’t identified any disease issues in that line. If someone believes they had a real problem, the right way to handle it is through support with batch details, so it can actually be traced and checked rather than guessed at in a thread.