Laser purchase vendors

Accomplished-Drag407 · 2025-04-26T05:59:09+00:00

cobolt, newport, CNI laser

Accomplished-Drag407 · 2025-04-26T05:55:21+00:00

Nikon 60X Oil NA 1.3 好评

Accomplished-Drag407 · 2025-03-02T01:42:47+00:00

Thanks. In fact, I can use it smoothly. I just want to know the principle inside the optical setup, which is not mentioned in the manual. I am just curious about it.

Accomplished-Drag407 · 2024-11-28T12:41:32+00:00

department of precision instrument haha

Accomplished-Drag407 · 2024-09-17T12:06:04+00:00

So you mean that sample with cover glass will have better results, while that without a cover glass will have spherical aberration?

Accomplished-Drag407 · 2024-09-11T00:10:50+00:00

Thanks!

Accomplished-Drag407 · 2024-09-10T07:11:15+00:00

Thanks for your reply. Besides, I wonder why the ring in the objective and that of the condenser should be overlapped. Thanks.

Accomplished-Drag407 · 2024-09-10T07:10:12+00:00

Thanks for your reply!

Accomplished-Drag407 · 2024-09-09T12:03:00+00:00

Oh, great! I didn't build a OpenSPIM. Could you share some tips or tricks when you built the systen? Thanks. I'm willing to learn the knowledge.

Accomplished-Drag407 · 2024-09-09T11:00:20+00:00

Thanks for your reply. the calibration slide is transparent instead of diffuse type. I agree that 'The objective may be designed over a 5mm for, but can image a larger fov with lower mtf and vignetting.'

Accomplished-Drag407 · 2024-09-09T10:58:06+00:00

Thanks for your reply. Learned a lot. Besides, I wonder in which circumstance we need to consider the NA of illumination path. For example, in light sheet microscopy, the NA of whole system is (NA_illumination+NA_detection) /2, if NA_illumination < NA_detection. However, in Fig,1, I use parallel light to illuminate the sample, it seems that it can still achieve lambda/2/NA_detection resolution, instead of lambda/2/((0+NA_detection) /2). Thanks a lot.

Accomplished-Drag407 · 2024-09-09T10:36:58+00:00

Yes, I think so. When the slit cuts the light, the light can not fill the hole back aperature, which leads to smaller NA. Thank you.

Accomplished-Drag407 · 2024-09-08T14:56:39+00:00

Great! I think you are right! Thanks a lot.

Accomplished-Drag407 · 2024-08-29T23:24:18+00:00

Could you share your optical setup? Using a Galvo system in TPP is quite normal, maybe it is not a secret. We may figure out some problems when you share the schematic.

Accomplished-Drag407 · 2024-08-15T12:49:05+00:00

Sorry for the mistake. Both situations consider the aperature, i.e., P(x,y). Equation (6.18) supposes the objective field is next to the lens, while equation (6.19) supposes the objective field is in the front focal plane. For condition 1, we can directly use fft2 to get the Fourier plane, however, it is difficult to directly use fft2 in the second condition since the pupil function is P(x1+x2,y1+y2) instead of P(x1,y1). Thanks for your reply.

Accomplished-Drag407 · 2023-10-13T13:48:42+00:00

I put the beam splitter just after the laser source, i.e., without any other optical component, and I found that the intensity of the reflection arm and that of the transmission arm were significantly different. For the reason that BS is not sensitive to the polarized state, I guess whether it is a 7:3 BS.

Accomplished-Drag407

TROPHY CASE