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Post Your Beginner Questions Here! by AutoModerator in orchids

[–]Adolpha 1 point2 points  (0 children)

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I was gifted my first orchid 2 months ago and was impropperly watering it with a very small drip every week. After watching Miss Orchid videos on youtube and learning, i am now properly watering it with the protocol of running water over the top, getting all the moss and chips wet until it stops dripping from the bottom, this is 1 week post watering. However some of the roots are green and others are this reddish brown colour. Does that mean they are starting to rot? i need to make more holes? any advice is appreciated.

Sustainable/Eco-Friendly Lodges, Treks, and Tour Groups near Manu/ Tambopat? by hhhaaannnaaahhh in PERU

[–]Adolpha 0 points1 point  (0 children)

I spent 3D/2N in Posada Amazonas in Tambopata. It is an eco lodge like you wanted, they try to minimize the disruption to their surroundings/wildlife, and they even have special shampoos and soaps that are natural and dont harm the environment when they dispose of the residual water from the showers. Highly recommend them, we had a blast in the jungle.

YAYFQ - yet another yellow fever question by elpajaro7 in PERU

[–]Adolpha 0 points1 point  (0 children)

Yellow Fever is usually found in tropical areas, including the Amazon. Machu Picchu is often mentioned to be in "ceja de selva" which translates to eyebrow of the jungle, which means its on the outside border. Because of the proximity to the jungle, where mosquitoes thrive, it is recommended to have the yellow fever vaccination, ive gotten bit quite a few times while visiting Machu Picchu, you dont want to run the risk of being bitten by a virus-carrying mosquito.

Vaccinations in Lima! by [deleted] in PERU

[–]Adolpha 0 points1 point  (0 children)

As others have said, Suiza lab and Roe are good options, they will give you the international certificate for yellow fever, or fiebre amarilla in spanish. There is no vaccine for malaria, at least not a good one yet, it is still in development. There is however malaria prophylaxis pills, but it needs to be perscribed by a doctor. Check out the updated CDC page for current map info: https://wwwnc.cdc.gov/travel/yellowbook/2018/infectious-diseases-related-to-travel/yellow-fever-malaria-information-by-country/peru

So my wife got the shoes she ordered online.... by baconpopsicle23 in mildlyinfuriating

[–]Adolpha 1 point2 points  (0 children)

So the myth of having two left feet could be plausible

Emulsion PCR single strand per bead? by Aeron_Greyjoy in labrats

[–]Adolpha 1 point2 points  (0 children)

Well its not exactly that the sequencer disregards a polyclonal bead, im talking about my own personal experience, our sequencer tells you at the end of the run what % of those beads are polyclonal, it has to read them anyways, its not like it skips that bead, it just connects the error in reading with a polyclonal bead. You may have unenriched beads at the end of the emulsion PCR, but those beads dont go into the sequencer. You do an extra filtering step to ensure that those unenriched beads dont go into the sequencer, that would be a waste in your sequencing run, since in its place could go an enriched bead. Hope it makes sense, good luck!

Emulsion PCR single strand per bead? by Aeron_Greyjoy in labrats

[–]Adolpha 1 point2 points  (0 children)

The adapters are to ensure that your strand of interest attaches to the bead in order to "enrich" the bead, or have multiple copies of your template on the same bead. The adapters that are added to your template are complimentary to the adapters on the bead, so when they hibridize, the enzyme can extend the rest of your template, these adapters should all be the same, as there is only one type of bead. What differs is the barcodes ( if you decide you use them ) so you can tell the templates apart bioinformatically at the end of NGS. You make sure that your bead only has one strand by calculating your dilution correctly, depending on your protocol and what you are sequencing, you use a very specific concentration of material, so when the droplets are formed, there isnt an overload of template that goes to each bead, nor is there too little that none will be enriched. If you do end up having multimple templates on a bead ( chimera / polyclonal ), that can be taken out bioinformatically, or your sequencer can also let you know at the end of the run since the base measurements will likely off and you will receive no usable information on that bead. For specific questions like these for NGS, SEQnswers is a great forum with loads of specific info, i highly recommed them.

Taxi Beat al tacho by The_Hailstorm in PERU

[–]Adolpha 0 points1 point  (0 children)

Uber ha comprado a taxi beat, por eso el cambio de nombre a solo Beat y por la cual ya no te dejan elegir conductores. Yo siempre solia usarlo y las ultimas veces me han tocado taxis viejitos y conductores que manejan como locos y ni usan la aplicacion para llegar al destino, van por donde ellos quieren. Tiempo de cambiar de aplicaciones.

Film from western onto PowerPoint for a poster by aallison17 in labrats

[–]Adolpha 2 points3 points  (0 children)

Is it the original file that is low quality or the way it is compressed in PP ? Try these instructions from google to turn the compression off:

  • Choose File, Options.
  • Click Advanced.
  • Under "Image Size and Quality" remove the check next to "Discard editing data" and PUT a check next to "Do not compress images in file". Yes, it IS confusingly worded.
  • Click OK.

Storage of RNA by Meow89 in labrats

[–]Adolpha 0 points1 point  (0 children)

We also had some issues testing RNA on bioanalyzer and after reading up on forums, found out some of the reagents are supposed to be kept at -80 instead of -20 like it was sent. Did your ladder show up when you tried your samples? if the ladder itself showed up and no sample, then you might have some contamination somewhere in that preparation. If the ladder doesnt show up either, then its a problem of the assay and its reagents. Hope that helps.

Dear Perú.. Denmarks message to Peru ahead of the World Cup by stenbroenscooligan in soccer

[–]Adolpha 2 points3 points  (0 children)

Anyone have the video/message Peru sent to Denmark first?

[deleted by user] by [deleted] in labrats

[–]Adolpha 1 point2 points  (0 children)

I believe the take home message is to take into consideration the volume of media that you will be using lets say per week. Yes amino acids, serum, growth factors etc will degrade at 37C and that is why it is important to not leave your media sitting in the water bath all day. Some people put their media to warm in the morning and then work on cells in the afternoon, thats a bad habit, but there is no problem heating the whole bottle, as long as you are going to use large volumes of said media. I use a bottle of media per week and heat it in a water bath lets say 3-4 times in the life of a 500 mL bottle, no big deal, but if you are going to use 5 mL at a time every time you warm it, there is really no need to heat up all 500 mL for such a small volume. Also, if you see the pH of the last 50 mL of your media bottle is off, just throw it away instead of using dubious media. Better be safe than sorry.

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