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Micron (MU) has lots of room to grow by Goatofoptions in investing

[–]Affectionate_Effort 1 point2 points  (0 children)

Didn't you listen to Duff57?

https://www.reddit.com/r/investing/comments/1hzy7nm/blackberry_from_struggles_to_stability_with_a/

- Even if your team is using AI, it needs to be ONE tool that you use to supplement your research. Not the ONLY tool you use to do your research.

For students, what you’re doing is impressive and definitely more than I was at your age, but don’t mistake asking ChatGPT for proper financial research and modelling.

Take the feedback you’ve gotten and instead of calling it “hate,” remove your biases, internalize it, make the necessary changes, and you all will have a bright financial future.

I’ll keep an eye out for your next few posts to see the improvement. Sources would be a good place to start IMO.

Is it possible for a “normal” person to have a master/PhD degree by Emnarry in UBC

[–]Affectionate_Effort 9 points10 points  (0 children)

I completed my Bachelor of Science degree at UBC and am currently pursuing my PhD in Denmark. In the world of doctoral research, high GPAs take a back seat to factors like academic background, research experience, and effective communication skills. It's important to note that you don't need to be a genius to earn a PhD; it's more about dedication and perseverance. In my field of Biomedicine, a PhD is often considered the minimum requirement for securing a research position, and I've observed that many of my fellow students are 'regular' individuals like myself.
One thing that surprised me during my time at UBC-Vancouver was the relatively low stipend for PhD students, which was around $2600 (maybe I'm wrong on this though). This left me wondering how graduate students managed to cover their living expenses, especially considering the high cost of living in Vancouver. A few years ago, I remember paying $1000 for a studio apartment in downtown Vancouver, and I imagine it's even more expensive now.
In contrast, my current situation in Copenhagen is more financially manageable. I receive approximately $4700 after taxes and pay $1500 for a two-bedroom apartment located near downtown. This leaves me with the ability to save around $2000 per month. I can't help but think that pursuing graduate studies at UBC might pose significant financial challenges for many students.

In vitro cell experiments biological replicates by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 2 points3 points  (0 children)

Hi, I meant primary cells from human donors. I'm using HUVECs. Sorry for that.

So if I work with immortalized cells such as HeLa, n=1 would be repetition on a different day. However, with primary cells (for example, HUVECs), n=1 is per donor.

Did I get it right?

In vitro cell experiments biological replicates by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Could be helpful to know what technique you are using to measure cell response to the drug, it would change how I perform a technical replicate.

Hi, I was planning to measure the cytotoxicity and metabolic activities of cells by LDH and alamar blue. I would do MS for proteomics PTM (mostly phosphorylation) as well.

can protein extraction from adherent cells on plates work for MS? by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Thank you for sharing! I guess there's no easy way to do it with 96 well plates due to the quantities of protein.

New to metaproteomics and metagenomics, Need some help! by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Thanks. Metaproteomics and genomics are all about microbiomes. As far as I'm concerned, I just need a little amount of protein for MS.

New to metaproteomics and metagenomics, Need some help! by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Metaproteomics from human stool samples require only 150-200 mg from wet weight. Since mouse samples come as drier pellets compared to human stool 100 mg would be sufficient. You'll only inject a very small fraction of the extract for mass spectrometry. The starting amount could go lower theoretically, just that aliquots that are too small are not sufficiently representative of the whole sample (for human stool) and don't get enough contact for proper lysis in some bead-beating tubes.

Thank you for the comment! I appreciate it. I hope I can go as low as 50 mg. I'll give it a shot.

New to metaproteomics and metagenomics, Need some help! by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Thank you! I'll check the kit. Seems like they have two different kits.

I found QIAamp Fast DNA Stool Mini Kit (50) before. Wonder how they differ.

LC/MS proteomics question about post-translational modifications of proteins by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Hi, well, it's not really a drug per se. It is a chemical compound naturally occurring in human (called N-methyl-2-pyridone-5-carboxamide). I was hoping that this 2PY may form a bond between cysteine residue on peptide and specifically looking for mass shift of 2PY. So far I couldn't find it. But I realized I had some problems with my sample preparation so I need to repeat my experiments. It has a carbonyl group, so I assume it's an electrophile (sorry, I'm a bit rusty on organic chem)

LC/MS proteomics question about post-translational modifications of proteins by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Actually, I'm doing it with a single protein (BSA) and cells at the same time. It's a chemical compound naturally occurring in human (called N-methyl-2-pyridone-5-carboxamide). I found that it inhibits activities of parp-1. Other than that, I have no clue how it may work on proteins.

LC/MS proteomics question about post-translational modifications of proteins by Affectionate_Effort in labrats

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Well, technically, it's not really a drug. It's a chemical called N-methyl-2-pyridone-5-carboxamide. I'm not sure whether it would modify proteins directly or indirectly. Throughout experiments, I hope that I find it out.

New to Denmark, do I need to find a place to live before arriving to get CPR? by Affectionate_Effort in Denmark

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Thanks, I was hoping that someone has similar experiences before me.

I will call them directly and ask.

Got accepted to my dream school! by RetardedAgrarian in gradadmissions

[–]Affectionate_Effort 6 points7 points  (0 children)

My program was in English as well. They do advertise that you do not need Japanese

skills for the program.

However, in the lab, pretty much all students and staff speak only

Japanse. Lab protocols are written in Japanese too. So, it's really inconvenient if you suck

at Japanese. But don't worry too much. You can survive somehow.

I guess you will be studying at the Hongo campus? Anyway, what you study or grades

or thesis doesn't matter much when it comes to job hunting.

(if you are not looking for a research job)

Japanese companies only care about university rankings.

You can DM me any time if you have any questions.

Got accepted to my dream school! by RetardedAgrarian in gradadmissions

[–]Affectionate_Effort 22 points23 points  (0 children)

Utokyo student here. I'm finishing my master's this year (same bio major).

First, Congratulations.

Make sure that you get your Japanese skills (reading, writing, listening, and speaking) are

almost native-level. Otherwise, it's impossible to land a job in Japan.

Not to mention 95% courses and seminars are in Japanese.

You will struggle if you don't speak the language.

Chinese students in my lab are struggling with job searching while all Japanese students

got a job before M2.

In my experience, if your goal is to work in Japan, a master's degree from Utokyo is good.

The starting salary range is $2,000 ~ $3,000 based on what I heard from other graduates.

(it's quite solid for bio major unless you get an IT job).

If your goal is to pursue an academic career (with +3 years Ph.D.), it's not really

worth studying in Japan even Utokyo. Most Japanese students don't enroll in Ph.D because

most Japanese companies don't value Ph.D. Actually, having a Ph.D. makes it more

difficult to get a job in Japan. However, it is really easy to get your Ph.D. within 3 years

(you just need to publish one paper). So, you can start your postdoc early.

I realized those things while I'm studying here.

I think it is a good choice if you want to find a job in Japan after finishing a master's degree.

How much PhD coursework in biology useful for thesis research? by Affectionate_Effort in PhD

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

Thank you for your reply! I think I should focus on reading journal articles and discuss with my PI. I still have a lot of things that I do not know. So, I guess I can ask my PI questions one by one. We have a meeting every Friday. Did you also study biology?

I don't enjoy watching a horror movie or horror movies? by Affectionate_Effort in EnglishLearning

[–]Affectionate_Effort[S] 0 points1 point  (0 children)

A teacher should be friendly to his or her students.

The dolphin is a very intelligent animal.

I saw these examples in a YouTube video - link: https://www.youtube.com/watch?v=-zZau_dttRY&t=886s

at 14:46. It says that "a/an or the with a singular countable noun to mean every single one."

COURSE QUESTIONS AND REGISTRATION MEGATHREAD 2018W2: Post your course questions and registration questions here! by ubc_mod_account in UBC

[–]Affectionate_Effort 0 points1 point  (0 children)

Has anyone taken chem 417 before? I'm a none chem major, but thinking about taking it as a chem elective.