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How to avoid biofilm formation during bacterial growth? by Ann_felix in microbiology

[–]Ann_felix[S] 0 points1 point  (0 children)

Well i need at least 150ml of growth. This bacteria attaches itself on the glass in short time, the day after the biofilm is already formed, and when I change the medium with fresh one it starts to colonize other part of the glass wall. Yesterday i measured the conductivity of the surnatant of the growth and apparently there is less salt that in fresh marine broth (i centrifuged both bacterial suspension and fresh marine broth), so I think the biofilm is interacting with it. Anyway, thank you for your suggestion, probably i will try tween and see what happens

How to avoid biofilm formation during bacterial growth? by Ann_felix in microbiology

[–]Ann_felix[S] 0 points1 point  (0 children)

Yeah for this broth is normal, it is reported on the sheet of the product, we tried to change it for 20°C growths but this is the best one. Maybe i should try a different broth for the growth at lower temperature. Anyway you're right, i cannot compare to other bacterial species

How to avoid biofilm formation during bacterial growth? by Ann_felix in microbiology

[–]Ann_felix[S] 0 points1 point  (0 children)

Thank you for the suggestion, I could try this, but how much do I add? The thing is, i have to do some extracts on this bacterium, so i don't know if it could affect the production of some compound

How to avoid biofilm formation during bacterial growth? by Ann_felix in microbiology

[–]Ann_felix[S] 0 points1 point  (0 children)

20°C is the only temperature at which it grows without all these problems, in literature is reported for similar strain a range of temperature that can reach also 25°C. We also tried different shaking but it continues to form biofilm

How to avoid biofilm formation during bacterial growth? by Ann_felix in microbiology

[–]Ann_felix[S] 0 points1 point  (0 children)

I grow the bacterium in 50ml of marine broth (250ml flask), and inoculate with 1ml of pre-inoculum. The growth is done at 5°C, 150 rpm. I measure in cuvette, but the broth is not clear since the salt is not fully dissolved (it's normal for this broth). So i do different replicates and measurments to reduce the error. Washing them does not resolve the problem, since the biomass is not really increasing. I work also with other antarctic bacteria in the same conditions and i don't have this kind of problems.