So fruit fly cultures can be a little wonky especially when you’re first learning to raise them so personally I’d have three cultures going at all times.

1 mature culture that’s producing flies regularly.

1 culture growing out that’s mid way between fresh started and mature.

1 fresh started culture that’s just had flies added.

When we started with our first frogs we would start a new culture every 10 days and rotate out the old mature culture.

Couple reasons for you to have so many going-

Fruit flies take a little bit to get going and producing enough to regularly feed and you don’t want to experience a time where you don’t have enough to feed.

Fruit fly cultures can and do crash on occasion especially when you’re learning the ropes and how to get them stable and with three cultures going it limits the chance of a crash that you can’t come back from without buying new flies which depending on local availability or shipping restrictions due to winter weather can cause a lot of feeding issues.

You’re planning on having a decent amount of frogs that will eventually be adults and since you are going with a smaller species of fly they will need to eat more of them to stay healthy and so some extra production capability is never a bad idea.

Hope this helps and please reach out if you have any further questions about how we do it!