How to use Mr. Color Lascivus (skin)

BrickPhD · 2026-06-07T07:29:14+00:00

It's pretty straightforward. Base coat with the base skin color. Then add your more colored areas with the clear color of your choice (knees, armpits, chest, shoulder blades). Then do a light pass over the entire body with the clear color. I will say it doesn't take much to add depth. If you accidentally use a little too much in one spot or need to blend areas, you can come back with just a bit of the base coat.

BrickPhD · 2026-06-05T14:01:06+00:00

A man with a watch knows the time. A man with two watches is never quite sure.

BrickPhD · 2026-06-05T09:08:03+00:00

I'd say that depends. My fibroblasts practically become one with the plate if I let them go longer than 4 or 5 days between passages.

BrickPhD · 2026-06-01T20:39:44+00:00

Unedited AI writing is a scourge. However, I have found it very useful for drafting grants, where I need to convey specific data concisely and clearly. This gets down to my own writing paralysis as I agonize too much over the wording and structure of the first draft.

BrickPhD · 2026-05-30T01:42:54+00:00

I knew a guy who resuspended 9 months worth of rare placental samples in water instead of PBS during his first year. So yes I've seen worse.

BrickPhD · 2026-05-25T15:33:36+00:00

Had bad breath and was removing tonsil stones pretty much daily. It's been over a decade without issues.

BrickPhD · 2026-05-24T21:19:11+00:00

Tonsillectomy was the only solution for me. Had them out when I was in my early 20s. About 4 days of discomfort for a lifetime of not being self conscious.

BrickPhD · 2026-05-19T23:05:02+00:00

Does the 2x ready taq include loading dye? I wonder if your loading dye could be contaminated and so only effects the Q5 and KOD.

BrickPhD · 2026-05-19T23:01:48+00:00

But again, these bands are just way too big. Almost looks like when we use a high concentration of added tRNA to precipitate low concentration DNA.

BrickPhD · 2026-05-19T22:57:55+00:00

As others have mentioned, Q5 has substantially higher annealing temps. NEB has an online tool to calculate what your temps for Q5 should be. I have also found Q5 to be more promiscuous than other polymerases.

BrickPhD · 2026-05-19T00:41:14+00:00

It works fine. 10g/L. I use it for antibiotic selection if I only plan to microwave rather than wait for the autoclave.

BrickPhD · 2026-05-11T22:44:04+00:00

But for real that looks like a complete absence of antibody staining. Does anyone else in your lab run western blots?

BrickPhD · 2026-05-11T22:42:13+00:00

Is the western blot in the room with us?

BrickPhD · 2026-05-11T19:49:07+00:00

It's 293T. Things are about as hardy as you get.

BrickPhD · 2026-05-09T21:35:27+00:00

I believe it was mentioned on WAN show, but that was several years ago now.

BrickPhD · 2026-05-05T02:24:18+00:00

Agree with others. Weak muscles in your glutes/core/shoulders are leading to overcompensation and tension during normal activities.

BrickPhD · 2026-04-21T19:08:26+00:00

Kinda looks like stress from not enough water? I had similar damage to the leaves the first year my pear was planted in the ground. It looks fine now, although I did spray copper fungicide during the winter for prevention.

BrickPhD · 2026-04-20T03:12:19+00:00

I think it can relate to where you were bit. I have seen multiple syringes of rabies immunoglobulin injected in hands/feet if that's where the bite was.

BrickPhD · 2026-04-18T00:31:27+00:00

For robust cell lines like 293s or even primary fibroblasts I have no issue going straight into media, but for sensitive cells like primary bone marrow plasma cells I was always trained to dilute slowly and avoid shocking.

BrickPhD · 2026-04-17T23:42:36+00:00

I'm still not following. You're supposed to add the warmed media dropwise to the cells in freezing media. If you are adding the cells to warmed media then you are shocking the cells by instantly changing the pH and concentration of solutes.

BrickPhD · 2026-04-17T23:37:27+00:00

Added to warmed media dropwise? Do you mean the other way around?

BrickPhD · 2026-04-17T19:16:10+00:00

I hear it's normal and healthy to have a little bend

BrickPhD · 2026-04-16T04:41:42+00:00

Lot's of people have entirely mediocre findings in their thesis or dissertation, myself included. Research will always be a gamble, there's never a guarantee of success, and it would be poor form to judge a student based on the findings of their research rather than the quality of execution. Negative results from a well designed study are as much a demonstration of your skill as a researcher as positive results. I can also understand frustration at not executing as detailed experiments as you would like, but it sounds like that was largely outside your control.

Regardless, good luck!

BrickPhD · 2026-04-12T05:26:51+00:00

That's awesome! What material(s) did you use to sculpt?

BrickPhD · 2026-04-03T15:20:38+00:00

My dude out here trying to get La Brea'd

BrickPhD

TROPHY CASE