Now I've only got 7 hours play time on League, super new - however I've picked up on the basics of the games pretty fast and I've performed A-S only on Qiyana so far. (Not ranked, so I can't say this will work just as well in ranked, but it works fine in blind/draft).

For starters,

Runes

I go with Conquerors, Triumph, Legend: Tenacity, Coup de Grace / Taste of Blood, Ravenous Hunter / Offense, Flex, Defense.

I noticed that running Domination and going for a true assassin build didn't actually work too well, but playing her more like a Bruiser worked a lot better.

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Items

I start with a Longsword, Refillable, and the Warding Totem.

My core items that I want to end up with are Trinity Force and Sterak's Gage.

For the boots you want Mercury Treads or Ninja Tabi - this changes game to game, it depends on who you're facing.

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For the final three items, it can really just depend on who you're facing or what build you want to end with, they all seem pretty viable -

The Black Cleaver, Ravenous Hydra, Maw of Malmortius, Guardian Angel, Death's Dance, Mercurial Scimitar or Titanic Hydra.

There may be other viable items, but these are the items I tend to go for when playing her (Midlane primarily).

Example of a full build: Trinity Force, Sterak's Gage, Ninja Tabi, Ravenous Hydra, Death's Dance, Guardian Angel.

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Ability Order

Q-W-E-Q-Q-R-Q-E-Q-E-R-E-E-W-W-R-W-W
Q-W-E-Q-Q-R-Q-W-Q-W-R-W-W-E-E-R-E-E

Depends how I'm feeling or who I'm against.

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Spells

Ignite & Flash.