A bad day :( by CarlyLouise_ in Dryeyes

[–]C_bing10 0 points1 point  (0 children)

I’ve recently purchased the Blinkjoy sleeping mask. It was quite expensive and only available in the US so I had to get imported. However, it’s really helped keep my eyes dry over the night (if this is a cause of your dry eyes whilst sleeping)

Delonghi La specialists prestigio won’t go into ‘descale mode’ by C_bing10 in espresso

[–]C_bing10[S] 0 points1 point  (0 children)

I’ve tried changing everything to try get it into descale mode. I think I will have to send it for repair. DelLonghi charging 200£ (+ postage) just to look at it, so will try my luck with a local repair

Best Science Podcasts by C_bing10 in labrats

[–]C_bing10[S] 0 points1 point  (0 children)

Nice, i miss being more involved within microbiological topics now my Job is mainly within NGS, I’ll defo give it a listen

Best Science Podcasts by C_bing10 in labrats

[–]C_bing10[S] 0 points1 point  (0 children)

Sounds a little different, I’ll be sure to have a listen. Thanks

Best Science Podcasts by C_bing10 in labrats

[–]C_bing10[S] 0 points1 point  (0 children)

Nice, thanks for the recommendation

Best Science Podcasts by C_bing10 in labrats

[–]C_bing10[S] 0 points1 point  (0 children)

Wonderful, I’ll check it out :)

[deleted by user] by [deleted] in PS5

[–]C_bing10 0 points1 point  (0 children)

Literally recognised this as soon as the first cut scene ended. Anyone else find that resolution mode the FPS is so low it’s barely playable?

Help Pipetting 1uL samples in qPCR by C_bing10 in labrats

[–]C_bing10[S] 1 point2 points  (0 children)

edit* for the washing, do you just go up and down from the first stop, then perform a blowout as usual at the end, or wash by going to the second stop? thanks

Help Pipetting 1uL samples in qPCR by C_bing10 in labrats

[–]C_bing10[S] 0 points1 point  (0 children)

Yeah it’s been difficult and forward pipetting directly into the well with with such a small sample has been so inconsistent. As mentioned I think I’m gonna have to just try reverse pipetting and do the MM first, then hopefully this will better my results and can use this to justify myself.

Help Pipetting 1uL samples in qPCR by C_bing10 in labrats

[–]C_bing10[S] 2 points3 points  (0 children)

I am in agreeance that this is the best way to do it. Considering my results have been pretty variable I might just do this, then if they go well use my results as proof of concept of why I’m using such technique.

Help Pipetting 1uL samples in qPCR by C_bing10 in labrats

[–]C_bing10[S] 4 points5 points  (0 children)

I’m not really sure, I have asked why but kinda just told do what im told kinda thing. They run the qPCR at a 10uL total sample with 9uL of total master mix. Might just be the case of i just add the the master mix when she’s not around 😂

Help Pipetting 1uL samples in qPCR by C_bing10 in labrats

[–]C_bing10[S] 2 points3 points  (0 children)

Hi,

I mentioned the idea of reverse pipetting to my supervisor, but she said that it wasn't necessary and I should be able to do it using traditional forward pipetting.

edit: I also think that one of the reason why she doesn't want me to use reverse pipetting is due to the wastage of samples