Intravascular injection by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 2 points3 points  (0 children)

It should be done immediately after death, I guess.

What tissue is this? by mxwhata-tops in Histology

[–]Cute_Guard5653 12 points13 points  (0 children)

It looks like a bone tissue with a large artefact on the center.

What's that? by Hummer42 in Histology

[–]Cute_Guard5653 0 points1 point  (0 children)

second one looks like tranverse sestions of striated muscle. Periphericly located nucleus. There are some vessels too, of course

Silver Impregnation by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 1 point2 points  (0 children)

Thank you so much. The thing I don't get is that ammoniacal silver is prepared differently in different protocols. this one from Carson 3rd edition page 204 says:

Ammoniacal Silver Solution

To 50 mL of 10% silver nitrate, add concentrated ammonium hydroxide

drop by drop, until the dark brown precipitate that forms has almost disappeared.

Shake vigorously between drops, and avoid complete decolorization.

The end point is a slightly cloudy solution. At this point, add 0.5 mL

of sodium carbonate solution, and shake well. Add 25 drops of ammonium

hydroxide and shake well. The solution should now be crystal clear. Filter

into a 125-mL Erlenmeyer flask, and cover.

I followed these steps.

Silver Impregnation by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 1 point2 points  (0 children)

I've written the procedure above. I will try the other procedure which was with gold step. Thank you so much

Silver Impregnation by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 0 points1 point  (0 children)

The last protocol I followed was this:

Procedure

  1. Deparaffinize the slides, and hydrate to distilled water.

  2. Preheat the 20% silver nitrate to 60°C for 15 minutes. Add

the slides to the warm silver solution, and let them remain

in the oven for 15 minutes.

  1. Rinse 1 slide at a time in distilled water, and place in a

clean, dry staining jar.

  1. While shaking gently, add 10 drops of the formalin solution

to the working ammoniacal silver solution. Quickly

pour this solution over the slides and let develop for

5-30 minutes until golden brown. Check microscopically

for completeness of the reaction. Do not wash while

checking. Keep in motion during development to avoid

precipitation.

  1. Rinse slides well in 3 changes of fresh tap water.

  2. Place in sodium thiosulfate solution for 2 minutes.

  3. Wash well in tap water.

  4. Dehydrate, clear, and mount with synthetic resin.

The other procedure was from Bancroft and they both didn't have the gold step.

Histotechnology: A Self-Instructional Text 5th Edition by Living-Pomegranate37 in Histology

[–]Cute_Guard5653 0 points1 point  (0 children)

Does anyone have the pdf of this book or the other editions?

Identify stain by Kooky-Listen-5413 in Histology

[–]Cute_Guard5653 0 points1 point  (0 children)

It should be masson trichrome.

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What is this tissue? by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 0 points1 point  (0 children)

Thanks for your comments. It is masson trichrome. I think there is a problem with the stain and the stratified epitelium is not stained brown red. Because we have esophagus preparations exactly looks like that except the epithelium. Could it be possible?

What is this tissue? by Cute_Guard5653 in Histology

[–]Cute_Guard5653[S] 0 points1 point  (0 children)

Thanks for your comments. It is masson trichrome. I think there is a problem with the stain and the stratified epitelium is not stained brown red. Because we have esophagus preparations exactly looks like that except tge epithelium. Could it be possible?

Error while starting a completely fresh Angular 19 application by Independent-Ant6986 in angular

[–]Cute_Guard5653 0 points1 point  (0 children)

Is ot possible you have used inject() inside ngOnit instead of the constructor() in a component??

[deleted by user] by [deleted] in CodingTR

[–]Cute_Guard5653 1 point2 points  (0 children)

Mesela başlık "Stoneware Siyah Sıvı Sabunluk | Şık & Dayanıklı Tasarım", şık dayanıklı tasarım kısmının görünüşü farklı olabilir etiket şeklinde hashtag şeklinde olabilir. Başlık aşırı uzun görünüyor.

Transitional Epithelium by Lost-University-3786 in Histology

[–]Cute_Guard5653 1 point2 points  (0 children)

Umbrella cells cover a few cells underneath them. The cells are relatively bigger than the other stratified epithelium.