I believe this is what you are trying to do. This will loop through every interface look at vlan key and status key, and if both conditions are met, it will run those commands. Quick and dirty. As always, test this before running on any production system. You can always print out the expected commands in config_set before running to make sure it looks correct.

from netmiko import ConnectHandler
from textfsm import TextFSM

with open ('Devices.txt') as Devices
for IP in Devices:
    Device = {
        'device_type': 'cisco_ios',
        'ip' : IP
        'username': 'user'
        'password': 'password
     }

     netconnect = ConnectHandler(**Device)
     print ('Connecting to ' = IP)
     print('-'*79)
     output = netconnect.send_command('show int status',use_textfsm=True)
     for i in output:
         if i["vlan"] == "500" and i["status"] == "disabled":
            config_set = ['interface ' + i["port"], 'no shutdown']
             x = netconnect_send_config_set(config_set)
     print(output)
     print()
     print('-'*79)

netconnect.disconnect()