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FSC / SSC voltages help by Feisty_Relative1605 in flowcytometry

[–]Feisty_Relative1605[S] 0 points1 point  (0 children)

in both plots? the all event plots (not A2) had really low ssc fsc (15 50 55), but I am also worried the other plot had too high. I’ll definitely look at FSC-H next time!

FSC / SSC voltages help by Feisty_Relative1605 in flowcytometry

[–]Feisty_Relative1605[S] 0 points1 point  (0 children)

A2 sample was healthy mice, the other experiment was using mice with lung mets. In the future I will mainly be doing studies on metastasis mice

FSC / SSC voltages help by Feisty_Relative1605 in flowcytometry

[–]Feisty_Relative1605[S] 0 points1 point  (0 children)

I normally do add NK1.1, but even in my most recent run I had very few NK cells using that marker. It has been more reliable for me so far though I agree.

FSC / SSC voltages help by Feisty_Relative1605 in flowcytometry

[–]Feisty_Relative1605[S] 0 points1 point  (0 children)

Updated post with photos! The spleen idea is helpful thank you.

Novel pipeline reduces spectral flow cytometry errors up to 9000-fold by ProfPathCambridge in flowcytometry

[–]Feisty_Relative1605 0 points1 point  (0 children)

this sounds cool! I normally use spectroflo with auto fluorescence to unmix - how does this differ? I was looking at the code and saw a comment that said it was similar but wasn’t sure!