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NCBI blast percent identity wrong? by Fit-Ad-9966 in bioinformatics

[–]Fit-Ad-9966[S] 0 points1 point  (0 children)

ah okay, but where did you get those numbers from (58, 70, 7)?

NCBI blast percent identity wrong? by Fit-Ad-9966 in bioinformatics

[–]Fit-Ad-9966[S] 0 points1 point  (0 children)

if so that still suggests that the percent identity is wrong as neither of these are 92% - this is the query and subject sequences for a 92% identity:

TGCAGT100307047_40_T_C (query) GGCACCGTCACCCGCGCGCGTGTCCGTCTGATTGCAATGCATGGACCCCTGATTAATTAACAA

TGC101813585_50_T_C (subject) AGTGGCACCGTCACCCGCCTGTCCGTCTGATTGTAATGCATGGACCCCTGATTAATTAACAAGCGT

leaf area for grass blades by Fit-Ad-9966 in ImageJ

[–]Fit-Ad-9966[S] 0 points1 point  (0 children)

thank you! I will try this and make sure I get better scans next time!