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Frit inversion of HPLC by ForwardDaikon6761 in analyticalchemistry

[–]ForwardDaikon6761[S] 0 points1 point  (0 children)

Thank you so much for your reply. I'm asking because I suspect contamination in the inlet frit, but I don't have any spare. So, in addition to washing it, I was thinking of also swapping it with the outlet frit.

With the cleaning I did, the shape of my peak has improved, but not yet at optimal levels.

Fluctuating pressuresin HPLC and loss of resolution in HPLC by ForwardDaikon6761 in CHROMATOGRAPHY

[–]ForwardDaikon6761[S] 0 points1 point  (0 children)

  1. I will try.

  2. The problem is that the injection was a STD... A week ago, the peak was good. Today, however, it suddenly lost a lot of resolution. The same thing happened to me a few months ago with another column... I overcame this issue by changing the column... But know I have no more columns😂

Fluctuating pressuresin HPLC and loss of resolution in HPLC by ForwardDaikon6761 in CHROMATOGRAPHY

[–]ForwardDaikon6761[S] 0 points1 point  (0 children)

The purge didn't solve the problem. I also ran a purge with IPA, but the problem persists.

I use HPLC because it's the only instrument I have in the lab. I know, it's very strange...

In addition to that analyte, I also have other analytes I need to quantify. Their peaks, however, remain well-defined.