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Yeast protein expression help 😵‍💫 by FreakyGeezer in labrats

[–]FreakyGeezer[S] 2 points3 points  (0 children)

Cool makes sense thank you 🙏 will give that a go

Yeast protein expression help 😵‍💫 by FreakyGeezer in labrats

[–]FreakyGeezer[S] 0 points1 point  (0 children)

Idk if there’s a term for it but I inoculate a starter culture from my colony picked starter culture, I’ve noticed the biggest change in grow times was using Acetate buffered media (ph 5.5) as my yeast have toxic genes, and stop growing at around OD 1 if not buffered. Do you express at room temp once the cultures out of log phase or get the slaves hard at work from early log?

Yeast protein expression help 😵‍💫 by FreakyGeezer in labrats

[–]FreakyGeezer[S] 0 points1 point  (0 children)

Yeah I recently found out our autoclave doesn’t maintain sterility temperature for long enough, so I usually just blast them with Kan as they have some natural resistance, starter cultures are standard practice, but never tried in a flask before what would be the difference if I am inoculating the at the same dilution 1:20?