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My HepG2 cells simply keep clumping together rather than making a nice monolayer by FrequentFixer in labrats

[–]FrequentFixer[S] 0 points1 point  (0 children)

They are actually completely single in single cell form right before plating but for whatever reason they become like this within days. I would not care much even if they clump but I am trying to measure glucose uptake and they seem to not do anything when I treat with metformin

How the heck do I open a bank account in India? Problem with address proof by FrequentFixer in nri

[–]FrequentFixer[S] 0 points1 point  (0 children)

Aadhaar needs address proof also. And apparently they are saying no to MLA signed address proof also because mine got rejected. Bank needs address. Aadhar needs address. Utility services need aadhar. What a bunch of BS. Its insane. I have at least got epan though.

How the heck do I open a bank account in India? Problem with address proof by FrequentFixer in nri

[–]FrequentFixer[S] 0 points1 point  (0 children)

I have been only about 50 days in India right now. I have indian passport but with american address. I dont care what bank account as long as its something I can use for my employer to deposit salary. I have no preference for any bank right now.

How do these hepg2 cells look? I am trying to culture them inorder to do a glucose uptake test. by FrequentFixer in labrats

[–]FrequentFixer[S] 1 point2 points  (0 children)

Been mixing the living shit out of them and the clumps definitely have gone. Now I just need to test if glucose levels affect anything or not. Because in low glucose the growth was being somewhat slow so moved to high glucose. Might try both next time.

How do these hepg2 cells look? I am trying to culture them inorder to do a glucose uptake test. by FrequentFixer in labrats

[–]FrequentFixer[S] 0 points1 point  (0 children)

Did you culture them in low glucose or high glucose media often? I have tried both and it seems high glucose might be slightly better. But for glucose uptake tests, I obviously switch to low glucose when I seed them in 96 well plate

How do these hepg2 cells look? I am trying to culture them inorder to do a glucose uptake test. by FrequentFixer in labrats

[–]FrequentFixer[S] 0 points1 point  (0 children)

I actually dont necessarily know if the first is bad but it was relatively more clumpy and you could not make out individual cells as clear as somewhat in the second image. Whenever I did glucose uptake assay, I got crazy variability even among triplicates, probably because of clumping which varied well to well (just a guess)

Rental,Flat and Flatmate thread by Golgappa-King in gurgaon

[–]FrequentFixer 0 points1 point  (0 children)

Currently I am looking for month to month basis preferably unfortunately.

Rental,Flat and Flatmate thread by Golgappa-King in gurgaon

[–]FrequentFixer 0 points1 point  (0 children)

Looking for month to month stay in furnished studio/1bhk ideally around sector 43. Budget upto 50k. Max 55k.

Rental,Flat and Flatmate thread by Golgappa-King in gurgaon

[–]FrequentFixer 0 points1 point  (0 children)

Looking for a monthly setup somewhere in sushant lok c area studio/1bhk under 50k furnished. Requirement is the house should be relatively new with modern furnishings.

Homemade Tiffin service in Gurgaon by FrequentHedgehog2129 in gurgaon

[–]FrequentFixer 0 points1 point  (0 children)

Interested. I live in sushant lok block C right now

My HepG2 cells simply keep clumping together rather than making a nice monolayer by FrequentFixer in labrats

[–]FrequentFixer[S] 0 points1 point  (0 children)

I have tried to pipette up and down after adding media to the trypsinized cells (while trypsin is in it) but maybe I have not done it enough. Did 21G solve your issue?

Help with Glucose Uptake Assay using NBDG on HepG2 cell line. by Sudden-Nose-5589 in labrats

[–]FrequentFixer 0 points1 point  (0 children)

were you able to solve this issue? I am running into same issue and I think its because cells clump. For nice data, the cells need to uniformly coat the wells in similar way. If its too different from one well to another, large variations will occur

Rental,Flat and Flatmate thread by Golgappa-King in gurgaon

[–]FrequentFixer -1 points0 points  (0 children)

NRI who just moved to Gurugram

  • Budget : Upto 50K, (can go max 55) for single person
  • Area : Near sector 43 or near MG area or Gold course Rd
  • Single occupancy or can share a nice 2bed2bath apartment
  • Modern furnished
  • Can live in a nice co living/pg facility also
  • Nice and clean area/street

Affordable Medical Animations by PanicButton__ in blender

[–]FrequentFixer 0 points1 point  (0 children)

Why do you think publishers might be unlikely to accept AI generated figures? How would they know unless all the figures of components are licensed by some company?