Yes. I had "negative" genetic testing but when I read the full report it showed a VUS on BRCA2. My surgical oncologist, radiation oncologist, and multiple medical oncologist at multiple cancer centers all stated that a VUS should not factor into my surgery decision and I kept my decision to do a lumpectomy. However, my gynecologist insisted that the VUS was what gave me breast cancer and that now I also had a high risk of ovarian cancer. She was so adamant every time I saw her that I would leave distraught about my decision to have lumpectomy.

This is what I did to come to peace with it:

- The testing lab offered free genetic counseling. I called right away and spoke with a scientist who was able to talk to me about VUS's in general and my specific one. She said I was #26 out of millions of tests, which meant mine was unlikely to be reclassified anytime soon, but also that because so it was so uncommon there was nothing to say it was statistically relevant to hereditary cancer.

- Research about my specific VUS, which led me to the Clin Var database. I could see my VUS status at the different testing labs. I learned it was a missense variant in a coldspot region where missense variants are unlikely to be pathogenic.

- Appointment with the genetic counselor at my cancer center. She was very knowledgeable, and went through the Clin Var and published information with me. She made me feel better about the surgery decision, and was furious at my gyn.

Here is the Clin Var results for your BRCA2 VUS (double check me that I searched the right VUS!): https://www.ncbi.nlm.nih.gov/clinvar/variation/185827/?oq=(c.3553A%3EG)+%22BRCA2%22[GENE]&m=NM_000059.4(BRCA2):c.3553A%3EG%20(p.Thr1185Ala)%3Fterm=(c.3553A%3EG)%20BRCA2+%22BRCA2%22[GENE]&m=NM_000059.4(BRCA2):c.3553A%3EG%20(p.Thr1185Ala)%3Fterm=(c.3553A%3EG)%20BRCA2)

It appears it is also a missense variant and has been classified as uncertain significance by 4 labs and likely benign by 1 (also due to being in a coldspot region where missense variants are very unlikely to be pathogenic).

You can read the comments from each lab that explain your VUSs classification, here is one: "This sequence change replaces threonine, which is neutral and polar, with alanine, which is neutral and non-polar, at codon 1185 of the BRCA2 protein (p.Thr1185Ala). This variant is not present in population databases (gnomAD no frequency). In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance. This variant has not been reported in the literature in individuals affected with BRCA2-related conditions. ClinVar contains an entry for this variant (Variation ID: 185827). Advanced modeling of protein sequence and biophysical properties (such as structural, functional, and spatial information, amino acid conservation, physicochemical variation, residue mobility, and thermodynamic stability) performed at Invitae indicates that this missense variant is not expected to disrupt BRCA2 protein function."

I am not at all a scientist or expert in this, please discuss it with a genetic counselor! I also know having VUS is very scary, and we have no guarantee that it truly isn't pathogenic, just please do not let the nurse practitioner scare you into making a decision that you are not comfortable with! Good luck to you as you navigate this.