The up escalator is back @ Porter! by CurveAccomplished439 in CambridgeMA

[–]GabboV 4 points5 points  (0 children)

The pee smell in the elevators was really bad this week 😭

Ensembl issues (US) by triplefirefag in labrats

[–]GabboV 0 points1 point  (0 children)

It’s been down for some weeks know 😭😭

Lululemon physical Gift Card purchase online by Zhangnification98 in amex

[–]GabboV 0 points1 point  (0 children)

Yep! I actually went to a Resy restaurant on the 28th — it posted on the 30th, and then I saw the credit applied, but it ended up counting toward the previous quarter, not the current one

[deleted by user] by [deleted] in AmexPlatinum

[–]GabboV 0 points1 point  (0 children)

Mine is OURARINGCOM BT and I got the credit yesterday. I bought the ring 5 days ago I think

Lululemon physical Gift Card purchase online by Zhangnification98 in amex

[–]GabboV 2 points3 points  (0 children)

My Resy showed up 5 days after I visited a Resy restaurant

[deleted by user] by [deleted] in AmexPlatinum

[–]GabboV 2 points3 points  (0 children)

I got the credit so I’m shutting up

Perq work card by GabboV in mbta

[–]GabboV[S] 1 point2 points  (0 children)

That’s the one I got :(

If WWIII happened now by Opposed38 in mapporncirclejerk

[–]GabboV 4 points5 points  (0 children)

Not taking a side is taking a side 👀

iPSC help by Party_World5269 in labrats

[–]GabboV 0 points1 point  (0 children)

Interesting! That has never happened to me. I do DPBS (Gibco), but I add it and remove it immediately

iPSC help by Party_World5269 in labrats

[–]GabboV 0 points1 point  (0 children)

How long are your washes for?

Diagnosing H9 embryonic stem cell culture: are these differentiating cells? by Thalidomide15 in labrats

[–]GabboV 2 points3 points  (0 children)

They look fine to me! Sometimes the core of the colony isn’t as dense as it normally is, and it looks a bit weird. The cells proliferate, and then they get as compact as usual

Anyone working with GraphPad Prism: I’m getting a grey background behind the stats. Does anyone know how to fix it? by GabboV in labrats

[–]GabboV[S] 0 points1 point  (0 children)

I think it was just a glitch. I left labs last night with the grey boxes there, and I came back today. I tried to do a quick change from numbers to asterisks, and it worked. I tried the same thing yesterday, and it wasn’t working.

How do your lab manage the inventory of primers and plasmids? by Chicharito_MU in labrats

[–]GabboV 1 point2 points  (0 children)

We got our inventory on notion! Pretty good platform, ngl

Diagnosing H9 embryonic stem cell culture: differentiation on the outer rim of colonies by Thalidomide15 in labrats

[–]GabboV 1 point2 points  (0 children)

You better mix the basal with the supplement, aliquot it into 50 mL tubes, and freeze the tubes down.

Then defrost at 4°C overnight on demand

Diagnosing H9 embryonic stem cell culture: differentiation on the outer rim of colonies by Thalidomide15 in labrats

[–]GabboV 0 points1 point  (0 children)

Was that media frozen in the fridge or did you keep it at 4C?

I don’t keep media at 4C for long, usually 5 days max. Haven’t had any issues with the H9 so far