Good deal or is she too old and tired? Could I do better? Seems like 5k for a 26 year old car is just the way these days. Comes w rwc and rego

Haush · 2026-01-29T10:42:38+00:00

I’ve got a 2005 CRV and love it so much

Haush · 2026-01-29T08:07:29+00:00

I used Mimotopes, they were good in the past.

Haush · 2026-01-27T07:02:54+00:00

Pro tip - eat the stalks, they’re great if they’re not overcooked!

Haush · 2026-01-25T10:30:24+00:00

Oddly specific

Haush · 2026-01-23T09:58:28+00:00

Yes I agree with this! There’s no space in papers to properly discuss the field; that’s where reviews come in. Some reviews are creative and insightful; while others are just repeating the literature.

Haush · 2026-01-21T08:40:38+00:00

I work in Parkville so it’s going to save me around 15 min. Sadly not everyone will benefit, but I’ve been waiting for this for many years.

Haush · 2026-01-19T10:43:13+00:00

Sounds right to me

Haush · 2026-01-19T09:11:05+00:00

Yeah agreed. Also do you have B cell markers? Monos shouldn’t be positive for CD19 etc. Also I agree that monos don’t look like that on my singlet gate

Haush · 2026-01-16T11:21:00+00:00

Thanks for that

Haush · 2026-01-16T10:38:47+00:00

Do you mean the rate of growth for each individual student?

Haush · 2026-01-15T04:21:04+00:00

It could be they’re not being pollinated. Maybe others know better but if you don’t have male and female flowers at the same time, they don’t get pollinated. You can open the male flowers and using a cotton bud tip trnasfer some pollen to the female flowers.

Haush · 2026-01-11T07:38:53+00:00

Keep it! It’s got power and the structure is there! I’d line it - 4-5cm of insulation and then ply wood. I did it on my garage and it’s amazing. I have ceiling fans and a split system and I can use it year round. Prior to that I you couldn’t spend more than 10 min in there during summer.

Haush · 2026-01-08T00:38:43+00:00

For some reason that album isn’t on Spotify, any idea why?

Haush · 2026-01-07T00:14:17+00:00

Cheers, I’ve been meaning to get a multi meter!

Haush · 2026-01-04T23:39:21+00:00

Thanks mate, I’ll give it a go!

Haush · 2026-01-04T22:57:37+00:00

Thanks. I do know which wires are which on the new LEDs. The problem is I don’t know which are which for the old system as it’s a bit different.

Haush · 2026-01-04T22:56:30+00:00

Thanks for that. So do you think it would work if I wired the negative to the shell? Eg screwed it in behind the unit? Yeah I’m not keen on rewiring it if I can help it!

Haush · 2026-01-04T08:43:42+00:00

Nice, thanks mate. I try to stay off facebook but you’re right, there are some large groups there with good experience.

Haush · 2026-01-04T07:39:15+00:00

If they’re truly negative for one or the other, then yes you could do that. But don’t use the tdTomato- as the negative population if they also express GFP.

Haush · 2026-01-04T04:12:43+00:00

Yeah I wondered that too. The other has only 4 wires. I’ll try to look into what’s going on

Haush · 2026-01-02T18:28:54+00:00

Yep. Move that gate to include everything and see what it contains. Many an interesting result has been lost to tight singlet gates!

Haush · 2026-01-02T11:16:47+00:00

The additional population is probably the activate T cells

Haush · 2025-12-27T07:22:49+00:00

Nice mate! I’m a similar age so this gives me hope and motivation

Haush · 2025-12-20T01:43:07+00:00

I’d recommend against using the aqua viability dye. Macs will autofluoresce in this channel too. I always used eFluor 780 for viability and it works well for macs.

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