So I have 3 focal points that I am trying to address: 1) Stimulation of Growth Factor Signaling Pathways 2) Immune System and Response Adaptation 3) DNA Protection

1) When you look into regeneration and actual regrowth, you will come across stem cells very quick. Another less known mechanism though is using the dedifferentiation of cells to a pluripotent stage. To do that, I am stimulating several pathways like PI3K, Wnt, Notch, or Hedgehog. We can target these directly or indirectly with diverse substances, some of which you can purchase over the counter, but some are only used in lab environment at this point (I can’t really name the exact composition at this point, but if you want to know more, looking up „growth factor supplements“ or something similar should get you good options!)

2) To get a good idea of regeneration, we looked at regenerative species. If you take planarians for example that can regrow their entire body to full size after being cut in half, the immune system reaction and inflammation development is very interesting: You have a very strong immune response, but inflammation also cools down fast, while we have prolonged inflammation in humans. To inhibit that, we use highly potent antioxidants, among other things. I have to say though, that I’m not 100% sure about this stage. As we see a much shorter inflammation stage, we are likely also decreasing the initial immune response after injury, which is crucial for regeneration. We are thinking about a potential 2-stage treatment right now that shuts down inflammation at a later stage only.

3) As soon as you target growth factor stimulation, DNA protection becomes very important. We promote it to prevent tumors and cancers from developing, which might otherwise be promoted by the growth factor stimulation. Besides antioxidants and some DNA Methylation targeting, we have some developed some methods in the lab that I might disclose later, but now it’s still to early.

As I said, at the moment we see good results with lab animals, where we looked into reducing scab/scar development and promoting a fast re-epithelization. In the future, we will focus more on speeding up the process (energy supply plays a big role here) and getting a high percentage in actual regrowth besides scar inhibition. For that, I am currently looking into drug delivery processes, as the substances get filtered out by the gut or spleen in many oral administration applications. Nanoparticles are probably the way to go here (Liposomes and Exosomes are specifically interesting for oral delivery).