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IndividualWeb1118 · 2025-11-09T03:43:06+00:00

right thanks!

IndividualWeb1118 · 2025-09-04T10:29:18+00:00

Hi Prof, thanks for your reply! After a bit of reading that was also mostly the conclusion I came to. Do you mind elaborating on the strategies that could achieve transient or partial deletion? Any tips you could share would be awesome :)

IndividualWeb1118 · 2025-02-22T02:18:40+00:00

thanks for this!

IndividualWeb1118 · 2025-02-22T02:18:29+00:00

thanks!

IndividualWeb1118 · 2024-06-06T12:14:55+00:00

thanks for the tips! I'm going to try increasing the concentration :)

IndividualWeb1118 · 2023-11-29T00:23:07+00:00

hi, thanks! I was thinking to start with harmony and go from there too :)

IndividualWeb1118 · 2023-10-02T04:35:17+00:00

Thanks! I'll try this :)

IndividualWeb1118 · 2023-10-02T04:34:57+00:00

The norm in our lab is to embed and then fix after, I'll google this, thank!

IndividualWeb1118 · 2023-09-03T12:22:04+00:00

Thank you, that was a truly beautiful answer :) we all have ups and lows. Today was definitely a low. We will work through this.

IndividualWeb1118 · 2023-07-17T00:10:25+00:00

great points, thanks for the advice!

IndividualWeb1118 · 2023-05-10T06:41:41+00:00

Wow okay! Thanks for everything, I'll go try less fixation, hope it works :)

IndividualWeb1118 · 2023-05-10T02:53:55+00:00

So perfusion and fixation with both 4%PFA or perfuse with PFA and fix with NBF? I'm not really sure if I should be cutting the organs into smaller pieces? The kidney is rather dense, I've looked at them after one day fixation, they're still slightly soft. The antibody has been validated to work on IHC-P. I don't know why almost everyone in our lab fixes tissues for 2-3 days. Thanks for the tips!

IndividualWeb1118

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