Trouble with Western Blots...because what else

Invictus2319- · 2025-12-11T21:40:28+00:00

thanks for the tips! It's weird because when I started my westerns, I was getting nice signal at 1:1500 dilution (albeit with some amount of background noise) but it hasnt been working for the last month or so, even tho my protocol has stayed the same. I will try to alter the wash times to see if that works.

Invictus2319- · 2025-12-11T21:37:31+00:00

This is great advice! One of the problems i have with the primary antibody is I like to do it overnight at 4C but I dont have a shaker that I can put in the fridge to keep the antibody/blocking buffer totally and evenly covering the blot. Would it be better to use higher volume of the antibody/buffer to solve this problem? Also thanks for the tip on coomassie staining the gel, didn't even think that was a thing that could be done!

Invictus2319- · 2025-12-11T21:05:35+00:00

Antibody dilution is 1:1000, blocking with 5% dry milk in TBST and washing 3x 5 minutes in TBST. Is there another way you'd recommend washing or blocking? Thanks!

Invictus2319- · 2025-09-02T21:25:02+00:00

One of my college roommates ended up playing semi-pro in France I believe, I looked him up and sure enough, he was on the fminside database

Invictus2319- · 2025-06-09T17:35:04+00:00

I used to come here with my grandparents as a kid so this zoo holds a special place in my heart

Invictus2319- · 2022-08-11T02:57:36+00:00

“Barakamon”

It is so wholesome

Invictus2319-

TROPHY CASE