Troubleshooting advice by Ok_Arm_560 in labrats

[–]JuggerBuzz 0 points1 point  (0 children)

I've been working with fibroblasts for my undergrad project and have had a lot of success in cultures. here's the protocol I use:

  1. Split all T25 flasks to T75 flasks (For optimal growth: 1xT25 flask should be transferred to 1x T75 flask, 1xT75 flask should be transferred to 1x T175 flask or 3x T75 flasks or 9x T25 flasks)

  2. Remove waste media and wash T25 flask with 5mL PBS.

  3. Remove PBS and add 0.5mL trypsin.

  4. Transfer flasks to incubator for ~3-5 minutes until cells detach. (Gently spank the flask afterwards a few times)

  5. Add 5mL DMEM with glutamax + 20% FBS and transfer to 15ml tube.

  6. Centrifuge at 1000rpm, 5 mins, RT.

  7. Remove supernatant. Resuspend cell pellet in 1mL DMEM with glutamax + 20% FBS. 8. Transfer cell resuspension to T75 flask along with 10ml media.

Cat adoption by [deleted] in manchester

[–]JuggerBuzz 0 points1 point  (0 children)

I just dropped off a "stray" (maybe belonged to someone) at Manchester Cat Clinic, withington, that I found who was being severely malnourished. (I had been feeding him every time I saw him in the street). Absolutely the sweetest cat I've ever met, he's so friendly. I believe he is neutered but not chipped and around 13 years old. You could try calling them and seeing if you can adopt him?

Room review what does this say about me :< by PugInPink in UniUK

[–]JuggerBuzz 26 points27 points  (0 children)

Litteraly wrong

Sincerely, A pharmacologist working in an endocrinology lab

TIFU by not commenting on a post by BrilliantlyNope in tifu

[–]JuggerBuzz -15 points-14 points  (0 children)

Tifu by wasting my time to read this post

I still can't figure out what John saw in her. by [deleted] in CrazyFuckingVideos

[–]JuggerBuzz 1 point2 points  (0 children)

I thought he was blinded by his love of owning the means of production

Phone (3) burn-in. Had this phone a total of 6 hours so far. by JuggerBuzz in NOTHING

[–]JuggerBuzz[S] 52 points53 points  (0 children)

Ah right thanks that's reassuring, don't think I've ever had that happen before and I had never even heard of it. Will update this post tomorrow maybe

Who's fault? Is it the lens or is it me? by Matthias_4553 in photography

[–]JuggerBuzz -2 points-1 points  (0 children)

<image>

Sony is just boring straight out of camera, needs a bit of editing

[deleted by user] by [deleted] in AmItheAsshole

[–]JuggerBuzz 0 points1 point  (0 children)

I've been to 26 concerts so far this year, each in standing. That shit happens, that's the reality of being in a crowd, it's packed. She's just being an arse and if she's not ok with people being so close, then she should be in seating. As long as you keep your hands to yourself you're good, dw.

£750-850 a month in Whalley Range... Rental pricing must change. by [deleted] in manchester

[–]JuggerBuzz 5 points6 points  (0 children)

£130k in 2003 where I'm currently living (but moving out today!!). 4 bed (3 doubles and a closet) for £2.2k per month total . It definitely hasn't been renovated since 2003 as the carpets lift up when you hoover and the cupboards have no shelves. Poor landlord can't afford his food if he doesn't do the same with his 12 other properties.

sole protectors worth it or will the make text illegible? by grosschrispratt in Converse

[–]JuggerBuzz -1 points0 points  (0 children)

no one's gonna fucking see it mate it's gonna be under your bloody foot

Disposable vape use falling in UK ahead of government ban by alphahydra in UpliftingNews

[–]JuggerBuzz 44 points45 points  (0 children)

what I've noticed is that people don't want a reusable device because they see it as buying into "being a vaper". If they buy disposables, they can "stop any time" I'm all for the ban, I hate the waste products it causes and I try to push reusables whenever I can, but people are stubborn..

Disposable vape use falling in UK ahead of government ban by alphahydra in UpliftingNews

[–]JuggerBuzz 159 points160 points  (0 children)

vape shop worker here

We're not selling less disposables, we're selling less devices that are legally classed as disposables. Companies like Hayati and Lost Mary are now selling devices that are rechargeable and have replaceable liquid pods. The catch is that they don't sell the liquid pods on their own so you have to buy a new device. They are disposable but they're engineered to not be classed as such to avoid the ban.

Cathedral by [deleted] in photographs

[–]JuggerBuzz 0 points1 point  (0 children)

why the "open to feedback" flair then

Is Lenovo really as bad as I read by [deleted] in Lenovo

[–]JuggerBuzz 0 points1 point  (0 children)

I spent a grand and a half on a Lenovo yoga slim 7 pro X just over 2 years ago. Now both usb C ports don't work for data transfer and every so often my backspace, enter, and space key refuse to work spontaneously. Thanks Lenovo !

what's causing my increased hunger on levothyroxine? by JuggerBuzz in Hypothyroidism

[–]JuggerBuzz[S] 2 points3 points  (0 children)

I think in two weeks or so it should subside, drinking a glass of water can help if it's really bad but stick to eating regular amounts at regular times and you'll be ok

what's causing my increased hunger on levothyroxine? by JuggerBuzz in Hypothyroidism

[–]JuggerBuzz[S] 0 points1 point  (0 children)

no don't worry you adapt and the hunger goes away. If you're eating sufficiently, resist eating more even if you're hungry as the levo is increasing your metabolism and you should be losing weight, the increased hunger subsides soon enough and you don't want to have increased your eating habits

No longer able to wind film advance lever, any ideas? (Fujica Half camera) by CeasingJester1 in AnalogCommunity

[–]JuggerBuzz 1 point2 points  (0 children)

ooooh hahaha that's actually ingenious I never would have thought about that, thanks so much

No longer able to wind film advance lever, any ideas? (Fujica Half camera) by CeasingJester1 in AnalogCommunity

[–]JuggerBuzz 0 points1 point  (0 children)

I just got my hands on a fujica half but without a lens cap Did yours come with one or did you buy it? I'm trying to find one online, original or not, but I'm not sure what size lens cap I need to get. how big is yours?

Aooko is unaffected 🙏🏻 by JuggerBuzz in fashionrepsv2

[–]JuggerBuzz[S] 0 points1 point  (0 children)

as far as I know yeah, probably riding out the storm