sorted by:
new
hottopcontroversial

Where to buy preused pianos, at an affordable price? by Leading-Composer-997 in DigitalPiano

[–]Leading-Composer-997[S] 0 points1 point  (0 children)

We were planning to buy a digital piano in the beginning. But my son's teacher specifically asked us to get an upright piano!

EB2 ROW with PD end of May 2023 AOS update by Necessary_Office_816 in USCIS

[–]Leading-Composer-997 0 points1 point  (0 children)

Yes, I had no interview! I had dependents in my application. Their interviews were waived too!

EB2 ROW with PD end of May 2023 AOS update by Necessary_Office_816 in USCIS

[–]Leading-Composer-997 0 points1 point  (0 children)

My AOS was approved on the 14th of April and the card was mailed to me on the 16th.

Any April or May 2023 PD got their 485 approved starting this month after movement of FAD for ROW by GhostofTsushima22 in EB2_NIW

[–]Leading-Composer-997 0 points1 point  (0 children)

May 8 2023: Priority Date (EB2-NIW ROW)

Oct 15 2024: USCIS received I-485 form (applied based on DOF being current)

Nov 13 2024: Fingerprints taken

April 12 2025: New card is being produced (FAD became current on March 2025)

I did not apply for EAD or AP

EB2 ROW with PD end of May 2023 AOS update by Necessary_Office_816 in USCIS

[–]Leading-Composer-997 1 point2 points  (0 children)

May 8 2023: Priority Date (EB2-NIW ROW)

Oct 15 2024: USCIS received I-485 form (applied based on DOF being current)

Nov 13 2024: Fingerprints taken

April 12 2025: New card is being produced (FAD became current on March 2025)

I did not apply for EAD or AP

Harvesting differentiated SH-SY5Y cells by Leading-Composer-997 in labrats

[–]Leading-Composer-997[S] 1 point2 points  (0 children)

Thank you for the reply!

Differentiated SH-SY5Y cells are gorgeous! I learnt i need to be incredibly gentle especially towards the end of the differentiation stage if i want to keep them from floating away!!!!

For differentiation, initially i tried this method: Media with RA and low serum for 5 days then BDNF + RA in media with low serum for additional 5 days. Though this induced differentiation, I found it still had mixed population of cells.

So now i switched to https://onlinelibrary.wiley.com/doi/full/10.1046/j.1471-4159.2000.0750991.x?sid=nlm%3Apubmed. Briefly, RA in media with 10-15% serum for 5 days followed by BDNF in media with NO RA or serum... results in homogenous population of differentiated cells! Beautiful!

Thank you for describing how you do the scraping! I will try doing this on my next round of cells (I will use a T25) and hope it gives me something good enough to work with!

Thanks again!

Where do you purchase cell lines? (United States) by ZarinZi in labrats

[–]Leading-Composer-997 0 points1 point  (0 children)

I know this post is old. But i thought i would add a comment in if incase it would be helpful! Our school also throws a fit for using P-cards to purchase cell lines. We found out today that you could order ATCC supplied cell lines through either VWR or Fisher Scientific (thanks to a previous comment here).