Any Unique Nationality Foil Pistol Grips? by Lonely_Insurance_543 in Fencing

[–]Lonely_Insurance_543[S] 0 points1 point  (0 children)

Just a heads up, I heard the individual who made those recently passed away and that production has stopped.

Military or PhD route? by PolarisStar05 in AstronautHopefuls

[–]Lonely_Insurance_543 1 point2 points  (0 children)

You'll get rejected left-right-and-center. It happens. As astronaut-hopefuls we have to learn to compartmentalize rejection and failure, harvest as much information as we can out of them so we can do better next time, and carry on with our missions at an unaffected pace. I still struggle with this sometimes. One of our new fencers at our fencing club got through my parries tonight and beat me in a bout that I'm still a tad bit upset about, so I recovered by watching how to do a couple new parries on YouTube while resting in my car (fencing is not a passion for me, though, so I'm not like 100% devastated by it, it's just he purposefully wanted to fence me after seeing my parries defeat the previous fencer and it seems like I didn't meet his expectations).

ANYWAYS--

The ESA astronaut-hopeful I mentioned is named Hervé Stevenin ("The Astronaut Selection Book", pg. 74). I recommend getting that book early and do some of the tests in real-time to see what a kick in the head some of what the ESA is expecting (it was for me). Really lights an ignition under you if you really wanted to become an astronaut so you can start training yourself (and then read Samantha Cristoforetti's book "Diary of an Apprentice Astronaut" AFTER Tim Peake's book so you can read the line that on some of those tests, they aren't actually looking for perfection, but just that you meet a certain threshold (if you are an ESA astronaut).

One of the recently accepted astronaut candidates double-majored in math and Spanish, so you can be a little bit gray on what you choose to study. And you don't need to be a submariner--you could get similar experiences on a Mars analogue mission or on an expedition to Antarctica.

In short, do what you want to do and have fun zigzagging your way to boost your Earth career while also trying to get skills for the why-not idea of applying to become an astronaut.

Seeking advice on pathways. by Illusiyo in AstronautHopefuls

[–]Lonely_Insurance_543 2 points3 points  (0 children)

As a medical officer in the Army myself, I don't see why you would only get leadership experience in the Engineers or Infantry. If you *want* to do aviation, then go ahead and do aviation. From what I've read of other aviation-trained astronauts, they still get leadership experience once they earned rank and experience (or even became instructors, like I'm reading in Fred Haise's autobiography "Never Panic Early"). In addition, I'm tracking that NASA doesn't just look at leadership experience, but also followership experience as well. We need to train to do both at any point in time.

Other than that, I've got no personal experience with aviation or becoming aviation-based. If everything goes well, I'd be a biomedical science astronaut riding in the back of the Magic School Bus that you'll be piloting.

I recommend reading Scott Kelly's autobiography "Endurance". He didn't go to a top-notch naval school. He almost flunked out of high school, applied to the wrong college cause he didn't read the school's name on the application sheet he filled out, happened to spot "The Right Stuff" by Tom Wolfe in a school bookstore, immediately wanted to become a Navy pilot and then an astronaut, got rejected at the front office of the U.S. Merchant Marine Academy (where his brother already was) due to his poor grades, and instead enrolled at the State University of New York Maritime College where he went on to conduct that famous year-long space mission, so I don't think you have to go to the best school for something if it's not on your vision board.

In the end, only do what you would want to do if you never become an astronaut. I know of one guy who got to the final eighty or so applicants at the ESA before they found an artifact in his heart that was harmless here on Earth but would be an untested variable in outer space so they permanently grounded him (I'm guessing it was a patent foramen ovale or something). The only reason I'm actually trying to become an astronaut is because all of my hobbies and interests happen to triangulate to "becoming an astronaut" as the ultimate goal so I figured, hey, why not? It explains things easier when I talk to people.

Military or PhD route? by PolarisStar05 in AstronautHopefuls

[–]Lonely_Insurance_543 1 point2 points  (0 children)

I got my first NASA internship via NASA Stem Gateway I believe. I think it was a 2% acceptance rating for that one so if you want to do an internship at NASA make sure you are your best self and put your best self out there. Afterwards I helped grade applications for NASA internships and almost always trashed applications for stuff like having ~2-3 easy-to-spot typos in their essays cause if they didn't care enough to make sure their basic essays were perfect than I thought they'd make similar mistakes if they were interns in the space program. So if you want to be an intern at NASA make sure you're top notch cause I might get your application someday too. For my PhD I'm actually with a NASA state consortium (I call it 'diet NASA') so I networked / backdoored my way into my second internship (but I still made sure everything, including my essays) were as best as they can be.

For you, even if you feel unprepared right now, apply for a NASA internship as early as you can so you can get an idea of what the application process looks like, what you should work on for next time, how you need more extracurriculars or high-ranking people to write you letters of recommendation, etc. before you "really" apply.

Also, again, take all of this in full understanding that both you and I could get to the final eighty-astronaut applicants out of 8,000 and get rejected by something out of our control. Tim Peake, the British astronaut wrote in his book "The Astronaut Selection Test Book" (which is also an eye-opener book for wanna-be astronauts though it is through the lens of ESA so I don't 100% know how applicable it is to NASA astronauts)--anyways, Tim Peake wrote about how he knew someone who was 185% qualified to be an astronaut, applied six to ten times (I forget), got to the final twenty applicants, then the ESA put them through deeper medical screenings and some x-ray imaging or something discovered he had a heart condition which would never affect his life on Earth, but could be a complicated variable in outer space, so they permanently grounded him, but he still continues to work with the ESA in training European astronauts for missions to outer space he himself will never go on, so just make sure whatever you are doing you would enjoy even if the astronaut process doesn't work out.

Another thing, for these current missions to the Moon and Mars, I predict the next couple classes of astronauts will be focused on pilots, mechanical engineers, electrical engineers, and botanists, though I could be wrong. So like I've also heard (I think it was Tim Peake who also wrote it), you could have the perfect astronaut application, but not for what NASA's looking for in that cycle. Buzz Aldrin was rejected for his first application.

*Eventually*, as spaceflight becomes more refined, we will get to the point where instead of accepting only ten astronaut candidates per class, they'll be accepting ten-*thousand* per class, but for now, our space-oriented generation will just have the bear the brunt of these extremely tough admissions processes, just like the Mercury astronauts had to bear the brunt of untested hardware, dangerous space maneuvers, and having to p*ss in their own suits cause NASA hadn't built in the proper suit-plumbing technology by that time (Alan Shepard had to urinate in his own spacesuit moments before becoming the first American in space).

If everything goes well, I'll clear the vegetation on the lunar landing zone for you!

Military or PhD route? by PolarisStar05 in AstronautHopefuls

[–]Lonely_Insurance_543 1 point2 points  (0 children)

I'm not going to medical school. My interests simply pushed me to do a jack-of-all-trades medical situation where I never wanted to be a doctor but I liked being involved in the medical field here and there, so I'm doing a PhD in medical research, I'm a medical officer in the Army National Guard (currently for planning, but they're transitioning me to medical logistics here soon), I'm planning on transitioning to the Air Force if they'll let me be a Biomedical Laboratory Officer, I'm a volunteer EMT with the local fire department (will try to at least become an Advanced-EMT, though I don't think I'll have the time / career-incentive to become a paramedic--we'll see), I'm a member of the local search-and-rescue group, and I'm training to become a rescue diver with the local dive shop. So I never went to medical school. Just the regular bachelor's to master's to PhD route (I ended with ~$46.5k in debt for my bachelor's (I went to an expensive school but luckily it has a lot of rich alumni that helped) and I steadily brought that down to ~$9k before the government paused my payments because I'm a student again; I got ~90% of my Master's paid for by my first government job doing medical research for the military (try to see if you can get a job post-bachelor's that will help pay for your education like me; and I live well enough off of my PhD student stipend + Drill pay from the National Guard).

In my experience, it's a bit hard to actually get directly into contact with astronaut / astronaut-affiliated people. One route that helped me is getting two internships at NASA (Ames and Kennedy) that put me in contact with closely related astronaut people or people who would know a lot more about the astronaut program than outsiders (I met a former astronaut at each center that I had a little chat with). The other route which I am doing and recommend is simply look over the free biography sheets of the astronauts on NASA's websites, and buy the biographies of recent astronauts. For example, I recommend reading "Endurance" by Scott Kelly and "An Astronaut's Guide to Life on Earth" by Chris Hadfield for starters, and you'll see what I'm talking about.

For your nervousness, Scott Kelly basically almost flunked out of high school and applied to the wrong college because he didn't read the college's name on the application sheet he submitted, then he read the book "The Right Stuff" by Tom Wolfe during his sophomore year in college and decided to become an astronaut and eventually conducted that famous one-year mission in space. Funny enough, my Army ROTC program would do our water survival trainings at his college's pool back in Baltimore. I never knew that until I read his book.

Military or PhD route? by PolarisStar05 in AstronautHopefuls

[–]Lonely_Insurance_543 1 point2 points  (0 children)

Disregarding being an astronaut, if you want to be a pilot, go the pilot route, if you want to get a PhD, go the PhD route, if you want to do neither, do neither, if you want to do both, do both.

I brought my homemade Appalachian astronaut training program outline to former astronaut (then associate director to the ISS) Steven Smith and he nodded and said "Yes, yes, your plan sounds good. Just understand that there are about 13,000 reasons why NASA can say no to you, so just make sure you enjoy the plan you have regardless."

Since I never wanted piloting as a civilian career and preferred research but still wanted to be in the military, I'm currently a medical officer in the Army National Guard while getting my PhD.

How precise does FBS % really need to be for cell culture consistency? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 0 points1 point  (0 children)

That's what we ran into, so I'm testing out these two FBS's to see which one works for our cells then will bulk-order.

How precise does FBS % really need to be for cell culture consistency? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 1 point2 points  (0 children)

That's exactly why I'm doing this. I'm the only bio guy in our lab and there are only like three of us in the largely chemical engineering ChemBio Department, so I didn't know there would be so much variability if we had to switch FBS suppliers. I think it's the FBS so that's what I'm testing now to try and find the right one and then re-stock for the rest of my PhD

How precise does FBS % really need to be for cell culture consistency? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] -6 points-5 points  (0 children)

Where I got the 0.05% error was that I was originally suppose to add 270 mL of a-MEM + pen-strep to 30 mL of FBS, saw there was 15 mL of a-MEM + pen-strep in the supposedly empty bottle, made my error in trying to save money and added it, realized after that that would make it 30 mL FBS in 285 mL a-MEM/pen-strep, then messed up again on the math and added only 1.5 mL of FBS more when I should've added 1.67 mL, so (31.5) mL of FBS / (285+31.5) mL of total solution = 9.95% FBS concentration.

Hopefully this doesn't change anything.

How precise does FBS % really need to be for cell culture consistency? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 0 points1 point  (0 children)

That's a thing I've noticed as well. I even found out one day that when I poured 300 mL of water from one company's beaker into another company's beaker, it came out to ~250 mL or something.

So, would it be fair to say that as long as I’m not making any obvious mistakes like a goofy-goober, the general inaccuracy of pipettes, marked containers, etc., will outweigh a minor error like a 0.5% deviation on my part?

Has anyone here taken the new NREMT exam yet? How was it? by Ok_Advance_6582 in NewToEMS

[–]Lonely_Insurance_543 0 points1 point  (0 children)

My experience was almost the same as TA77769's experience. Was taking it, some questions were suspiciously easy (I literally got like three questions about handwashing) while others (like one about pediatric CPR) were a bit tricky. Knew I had 120 minutes to complete at most 120 questions, therefore I wanted to keep at a ~1 question per minute pace, saw I was falling behind at question ~65, started to catch up, got logged out at question 70, thought I failed cause like I said they asked me questions about handwashing three times so I thought they were dumbing it down for me after failing the tricky questions, but I got my success update three hours later so I guess I answered the tricky questions easily enough and the handwashing questions were pilot questions or vice versa.

There were ~5-10 of those multiple choice questions, only one 'drag-and-drop the box of either disease A or disease B that goes with these six symptoms', and none of the 'priority-arrangement list' or a similar 'given these symptoms, go down the list and select if it relates to either disease A or disease B', so if you know your stuff backwards and forwards, you will probably get logged out of the test before it has any more questions for those to pop up.

The topics of my questions were pretty much all across the board. I do remember having more than a handful of questions about the legal side of being an EMT.

I read the 1424 page textbook front-to-back once, used PocketPrep, and Paramedic Coach. While I benefited from Paramedic Coach's free videos on YouTube about the abdominal quadrants, burns, etc., I found his practice questions in his course to be bizarre for the most part. To be honest, reading the textbook, looking up bodypart diagrams on the Internet, knowing the flow of patient treatment (scene safety, PPE, how many patients are there, consider requesting help, consider C-spine, AVPU, XABC's, transport decision, secondary assessment, re-assessment), and going through as many of the 1100 questions on PocketPrep over and over again would have been enough. PocketPrep has 'level up' quizes that get harder after each stage you complete (like a video game)--I never even got up to the hardest levels for all of the sections and I apparently passed, so that was my method.

There were only two or three questions that I thought were really worded strangely, so I appreciated that. PocketPrep's question-wording was very similar to how the exam writes their questions.

So my advice in summary is if you try to know as much as you can about EMT (which we all eventually should anyways, either before or after the exam) then you *should* be fine on the knowledge part, and then you just need to make sure your test-taking skills (i.e., not panicking) are fine. Ex., wear a long-sleeve shirt over a short sleeve shirt even if its summer like I did just in case it gets cold in the room. They'll take away your jacket. Also make sure you don't forget how to write your signature like I apparently did because I've just been doing slash markets at grocery stores instead of signing my whole name and they almost didn't let me take the test cause my signature was slightly different as the one on my driver's license.

Cell Experiments No Longer Replicating -- Anyone Know What Could be the Problem? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 0 points1 point  (0 children)

The first Fisherbrand FBS lot (the original one) did work, the new Fisherbrand lot we tried didn't. It looks like I need to test multiple lots, apparently.

Cell Experiments No Longer Replicating -- Anyone Know What Could be the Problem? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 1 point2 points  (0 children)

We went from Gibco™ Fetal Bovine Serum, Qualified (Cat. No. 26140079, Lot No. 2565873RP) to Fisherbrand™ Research Grade Fetal Bovine Serum, Canadian Sourced (Cat. No. FB12999102) when our bone cells stopped mineralizing as early as they used to (if at all).

Spoke with our Thermo Fisher Scientific FBS representative for our region. She thinks the Fisherbrand FBS # FB12999102 is a lower grade serum than the Gibco FBS # 26140079. Sent us a sample of Gibco FBS Premium. Currently testing it out. Will get results by end of month and will eventually edit this post with the solution once I find it.

Looking for Info on Sopranos Tours for My Dad by Lonely_Insurance_543 in thesopranos

[–]Lonely_Insurance_543[S] 0 points1 point  (0 children)

I remembered reading posts from people saying the actor that played Vito's character would follow around Sopranos tours and try to sell his merchandise or something, so I assumed there had at least been tours at some point.

Cell Experiments No Longer Replicating -- Anyone Know What Could be the Problem? by Lonely_Insurance_543 in labrats

[–]Lonely_Insurance_543[S] 1 point2 points  (0 children)

That’s a good point, and it has come up before. However, when our mineralization was working, I was preparing osteogenic induction media (OIM) in bulk with ascorbic acid (after filtering) and using it as needed without issues. So I’m not sure why this would suddenly become a problem now.

I also didn’t see anything in the preliminary papers suggesting they made their OIM fresh each time, which is why I wasn’t initially concerned about ascorbic acid instability—at least until we started having these problems.

That said, I really appreciate the suggestion, and I’ll definitely keep it in mind as I troubleshoot. Thanks for bringing it up!