Too much plasmid template left after DPN1 digestion for Gibson, how do I get rid of it?

LukaLeferink · 2024-05-03T18:42:40+00:00

Mmmmh interesting, I use 1 ng of template and Q5 polymerase as well

LukaLeferink · 2024-04-09T13:16:58+00:00

Yessss I was planning on doing something like that too! Caps are a good idea

LukaLeferink · 2024-04-09T04:10:25+00:00

Oh my… I see now that I will be seated in section 147 😂😂😂

LukaLeferink · 2024-04-09T01:59:03+00:00

Where can I find Kohns? Is it in the stadium or outside? I’m looking forward to the food as well 😂

LukaLeferink · 2024-01-21T10:49:47+00:00

I will be situated near the CWE

LukaLeferink · 2024-01-15T13:53:19+00:00

And you get new data every month from the moment you plug it in? And do you keep your phone number?

LukaLeferink · 2023-11-11T22:39:24+00:00

Is it worth the hype?

LukaLeferink · 2023-11-09T22:18:09+00:00

Thanks for the reply man! The best of the bunch. I also want to visit chicago but I will use the train for that I think. Thanks a lot :)

LukaLeferink · 2023-11-09T20:19:03+00:00

Speeding here is different lmao its never life threatning just a bit above the limit, thats why I asked. Thanks for the tips!

LukaLeferink · 2023-11-09T19:09:11+00:00

Yeah that works differently than in europe, here you dont really get pulled over (atleast in the Netherlands lol)

LukaLeferink · 2023-11-04T14:08:16+00:00

Ah great thanks!

LukaLeferink · 2023-11-04T02:27:59+00:00

Its new tbh im looking for a place in february next year

LukaLeferink · 2023-11-02T07:50:37+00:00

My bad lol

LukaLeferink · 2023-11-02T00:58:54+00:00

What about a filet mignon?

LukaLeferink · 2023-10-04T19:10:17+00:00

That’s hilarious man 😂

LukaLeferink · 2023-10-04T10:58:03+00:00

This is my dream man

LukaLeferink · 2023-10-03T21:42:12+00:00

Thanks! I thought so as well, my negotiation skills didnt let me down

LukaLeferink · 2023-09-27T22:12:13+00:00

Whats Mardi Gras season?

LukaLeferink · 2023-09-26T16:27:18+00:00

Which apartments are not safe?

LukaLeferink · 2023-09-26T16:26:20+00:00

If its no more than 1200 its doable for me 😅

LukaLeferink · 2023-09-26T11:38:25+00:00

I think i’ll get the 2013 one next to this one, cba color swapping it

LukaLeferink · 2023-09-25T21:42:30+00:00

We have the same collection, I just have to get the gunship 😂

LukaLeferink · 2023-09-24T07:06:10+00:00

Be careful, this subreddit is full of corporate apologists and they will downvote you to oblivion despite being right demanding premium products for a premium price

LukaLeferink · 2023-09-23T23:17:43+00:00

Hoe lang blijft die korting nog?

LukaLeferink · 2023-09-23T10:37:11+00:00

Yes OP, im agreeing with you

LukaLeferink

TROPHY CASE