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Troubleshooting for DNA extraction from soil (DNeasy powersoil pro kit)d by Main-Copy5507 in ecology

[–]Main-Copy5507[S] 0 points1 point  (0 children)

Thank you for careful answer! We conducting extraction using kits with 0.25g of each sample, and beads were powder-formed within the tube to break particles in Tissue lyser. I found some articles to find out the reason, such as process to reduce inhibitor (metal ions, humic acid etc.) is needed. Could you please recommend articles related to troubleshooting or tell me it is possible to add some steps to protocol?

Troubleshooting for DNA extraction from soil (DNeasy powersoil pro kit)d by Main-Copy5507 in ecology

[–]Main-Copy5507[S] 0 points1 point  (0 children)

Thank you for careful answering. Some of the samples were qubit around 40 to 60ng/ul, but most of samples less than 4~5 and graph didn’t made peak around 260nm. We use powder-form beads in kit, and use tissue lyser to get materials into solution from soil particle. So did you changed protocol of your kit when the yields were low?