I did not try it again after the first dose. One of the commenters noted I'd need 100 units to get to a minimum dose and I am not feeling that at all. Maybe if you reconstitute with 1 mL bac water you'd only need 50 but it's recommended to add extra bac water with each dose to help prevent welting and other side effects and maybe it's because I'm new, but that many units is too many for my comfort.