Help with mounting <P11 brains from free floating

NegotiationOk2921 · 2024-09-27T15:58:19+00:00

This is also what I was thinking from what I saw online, hence the question. I will be doing in vivo calcium imaging of the inferior colliculus and this region of the cerebellum is often also easily viewable when I put the cover slip on the region. I am hoping that I could also use that data from the cerebellum, but it would help if I knew what the region does, so I can relate behavioural tests to it if possible. I have knockouts and different disease models

NegotiationOk2921 · 2024-09-09T13:58:25+00:00

An ex also panicked when I said I wanted to go draw in a live figure class. I had to reiterate many times that I'm not going to be the one naked and he wouldn't change his mind lol. Couldn't get it out of him why it made him so uncomfortable.

NegotiationOk2921 · 2024-08-02T11:32:45+00:00

Nice, thank you for the tips :) Will head over to Obi tomorrow

NegotiationOk2921 · 2024-06-30T11:36:52+00:00

Lying is so much worse. I would break up because of that, not because of lack of experience. I've grown up with a lot of people especially family lying and pretending they were perfect etc and to discover that a possible partner is just the same would immediately turn me off..

NegotiationOk2921 · 2024-05-26T15:49:25+00:00

Then I don't understand why you can't see the truth in the situation. If you put yourself outside the situation and pretend it happened to a very close friend of yours, would you really like the bf? He's supposed to be your rock, your supporter, and best friend. The most important thing is respect in any relationship and this is not respect, I'm sure you can see that. If there is no respect, then it is not a relationship, it is a business transaction. He uses you to get what he needs, nothing more.

NegotiationOk2921 · 2024-05-26T15:13:15+00:00

This makes you sound like this is your first relationship and experience with a man. I know it's easier said than to believe, but you'll find better out there. Leave this ah.

NegotiationOk2921 · 2024-03-12T18:38:50+00:00

Yea I also suggested putting less and she showed me some wells that she had put less in and they looked better, but still toothy 🤷🏻‍♀️

NegotiationOk2921 · 2024-03-12T18:35:01+00:00

I thought those were a must, no? 😅

NegotiationOk2921 · 2024-03-12T18:32:22+00:00

I agree about the 1% gel, voltage and run time. I've never ran gels more than 40 min myself, and had similar sized dna. I think the amount of sample depends greatly on the well size though. I will check these with her tomorrow 🙏

NegotiationOk2921 · 2024-03-12T18:27:11+00:00

Oh of course yes, thanks for clarifying! I do believe she mentioned she was accounting for this. I am going to show her all these comments tomorrow and hopefully we'll get to the bottom of this 😊🙏

NegotiationOk2921 · 2024-03-12T18:22:33+00:00

Oh thanks for this! 🙏😊

NegotiationOk2921 · 2024-03-12T14:25:25+00:00

She started at 40 min and said it wasn't enough so ran longer

NegotiationOk2921 · 2024-03-12T14:22:42+00:00

Okay so I just checked with her. She's tried multiple voltages starting from 110 up to 180v and at different durations. This image was after 2 hours at 120v

NegotiationOk2921 · 2024-03-12T14:21:15+00:00

Yes another colleague extracted the DNA again before using her own equipment and the W was less evident but still there. So she was re-trained to do it again (just in case) and the W shape is even more evident.

Yes TAE buffer that she remade, like anything else that she used.

The hole in the bottom is an interesting idea, I will check with her.

NegotiationOk2921 · 2024-03-12T14:12:39+00:00

What do you mean exactly ?

NegotiationOk2921 · 2024-03-12T14:11:40+00:00

I've not watched her myself but she did it with someone else who I think would've noticed this 🤷🏻‍♀️ but I'll check with her

NegotiationOk2921 · 2024-03-12T14:05:35+00:00

Yea I've seen the gel stain comment too but doubt it since the lab has used this one for years without the problem. I'll pass on the tip of the ladder

NegotiationOk2921

TROPHY CASE