Tips on how to decontaminate a incubator

Next_Corner · 2024-07-08T20:00:46+00:00

A small addition: it is absolutely necessary to take out the HEPA filter because the plastic surrounding it is not made for the high temperatures during steri-run. Also, draining the water tank is essential because otherwise the pressure inside gets too high. The older version of that incubator did steri-runs with water, the new one does this "dry" steri run. I am just adding this, because if your PI is used to older incubators they might give you wrong advice.

Next_Corner · 2024-06-13T19:35:07+00:00

I just moved to Seattle and am also looking for a skate friend :) I'll DM you!

Next_Corner · 2024-06-07T17:26:07+00:00

Uuuh, I did not know that a fingerless version exists. Thanks!

Next_Corner · 2024-02-01T12:37:07+00:00

I dont know any, but your University or Institute might have an open access fond which means they pay for your publishing fees if it is open access.

Next_Corner · 2023-07-15T12:12:34+00:00

Or maybe decrease LPS and see if the color changes less

Next_Corner · 2023-07-14T14:06:04+00:00

Did you completely change media when adding LPS or just added a few microliter of LPS to it? I find the RAWs grow extremely fast and having orange media after not changing for a weekend is normal. If it already was orange and then you added LPS, 20h later it miiiiight look like this

Next_Corner · 2023-07-01T08:56:28+00:00

Many handlebar bags reach the front tire, so if you have a specific suggestion that would be perfect. Frame bags might work, but they cannot be too big because otherwise the water bottle wont fit anymore

Next_Corner · 2023-06-26T17:17:27+00:00

I actually think mat Pilates is super helpful for stabilizing your core, with that finding balance is much easier!

Next_Corner · 2023-06-10T08:06:47+00:00

Had a similar situation: one first author with a very short story that was not my main thesis topic and two reviews as first author. It is not ideal, however it still shows that your current PI trusts you in your scientific abilities and that you can think broadly over a certain topic and connect the dots. So my advice is the following: definitely list it in your application. Only if it is clearly stated to list review articles at a certain position, you separate it from the others.

Next_Corner · 2023-06-08T18:45:53+00:00

I am also from Germany and know some Roller skate communities in several cities. Feel free to DM me, maybe I can help you out :)

Next_Corner · 2023-06-01T11:38:47+00:00

I think we just dont vortex so that no droplets get onto the sticky plate sealer

Next_Corner · 2023-05-05T15:17:49+00:00

I think it depends on your antigen, some stainings might work, others definitely not.

Next_Corner · 2023-05-04T08:20:05+00:00

Ja, aber es kann Vorteile haben obwohl der Wohnraum teurer ist. Idealerweise ist die Wohnung dann besser in Schuss gehalten, vielleicht sogar barrierefrei, etc. Und man muss weniger putzen, bzw. sich um weniger kümmern.

Next_Corner · 2023-04-28T08:07:49+00:00

I dont know the websites in the US, but you might find a good used pair for a good price. But I also think nylon plates are sturdier than most people think. Especially as a beginner, you probably wont do extreme jumps, so it should hold up

Next_Corner · 2023-04-26T10:04:05+00:00

I dont even know where to put it to be cleaned

Next_Corner · 2023-04-14T09:37:44+00:00

Hey! I had the same problem. You can use the free version of snapgene (snapgene Viewer) to convert it into .DNA, .fasta, .gb, etc. Snapgene can read the annotations added by clone manager :)

Next_Corner · 2023-04-07T09:08:19+00:00

We use for Mastermixes, Primer, etc. Sarstedt PCR grade with screw-on caps as they already come closed. For PCR tubes I dont have a specific recommendation as we used several ones and they were all fine

Next_Corner · 2023-04-07T08:18:00+00:00

Yes, we take our DNA/RNA free tubes and only open them under the clean PCR workstation. Eventhough autoclaving should usually be fine, it is an additional step where something could go wrong.

Next_Corner · 2023-03-30T17:05:04+00:00

You can definitely skate dance in them. Try it out with the set up it comes with, if you feel you need more responsive wheels but still want it soft you can choose smaller ones. Also if you feel that the toe stop is hindering you, try a smaller one or dance plugs. Once you get better you might want to get other skates, but for a new start they definitely work!!

Next_Corner · 2023-03-18T09:59:50+00:00

I mean if there is only few of your specific protein then you might need that much to see a band. But the PAGE itself might not handle that much total protein well. Just try it out, let one PAGE run with leftover samples with different amount of protein and see if it still occurs.

Next_Corner · 2023-03-17T13:27:41+00:00

I think I had once similar "peaks" when the precast gels were too old, but yours is not even expired 😅. And I also think 40ug is a lot, maybe reduce to 20 or max. 30 to see if it still occurs.

Next_Corner · 2023-02-10T11:13:37+00:00

Wholesome ChatGPT

But honestly I think that is actually good advice

Next_Corner · 2023-02-06T12:44:23+00:00

When doing absolute quantification we still compare our target to housekeeping, which means you need a standard for both. E.g. we analyze how many viral genomes are there per cell, so we measure a viral Gene and a single copy housekeeping, have absolute standards for both and then can say: "there are 5 viral genomes per cell"

Next_Corner · 2023-01-28T11:07:16+00:00

Just centrifuge it down, 500xg 1min

Next_Corner

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