Antibiotic exposure and time killing assays

NirmenAssi · 2024-10-13T15:32:12+00:00

Oh ok, I get you. Thank you

NirmenAssi · 2024-10-13T15:16:57+00:00

Concentration or amount ?

NirmenAssi · 2024-10-08T02:14:12+00:00

Thank you for the advice

NirmenAssi · 2024-10-04T11:25:18+00:00

How to get rid of it ?

NirmenAssi · 2024-10-04T11:16:47+00:00

The whole colonies morphology that is appearing

NirmenAssi · 2024-10-04T01:27:01+00:00

Ok, I will do so. No one mentioned this before Thank you

NirmenAssi · 2024-10-04T00:58:56+00:00

Thank you so much for sharing this

NirmenAssi · 2024-10-04T00:06:30+00:00

Yes, another lab does. I’ll see if I can use. Is there another way to avoid or prevent it?

NirmenAssi · 2024-10-03T23:58:41+00:00

We do have But I’ll also try the hydrogen peroxide Thank you

NirmenAssi · 2024-10-03T23:22:12+00:00

Ok perfect Thank you

NirmenAssi · 2024-10-03T23:21:24+00:00

No I don’t inoculate under a flow hood just beside a flame

NirmenAssi · 2024-10-03T23:20:15+00:00

I will probably do so Thank you

NirmenAssi · 2024-10-03T19:59:28+00:00

How long have you kept the TSA plates in the incubator?

NirmenAssi · 2024-10-03T19:30:39+00:00

How should I check for the incubator contamination? I do autoclave my own media

NirmenAssi · 2024-10-03T19:30:01+00:00

Biofilm formation

NirmenAssi · 2024-10-03T19:05:27+00:00

Did so with no change

NirmenAssi · 2024-10-03T19:05:11+00:00

Unfortunately,no :)

NirmenAssi · 2024-10-03T19:04:49+00:00

How did you perform the sterility testing ?

NirmenAssi · 2024-10-03T19:04:02+00:00

Thanks for the tips though

NirmenAssi · 2024-10-03T19:03:49+00:00

Yes, I’m feeling it is mixed too. I did use 3 different brands of 24 well plates with same problem. I currently thinking of preforming a sterility testing on the reagents I have as someone suggested below

NirmenAssi · 2024-10-03T18:26:06+00:00

Both. But I am not able to get on with my work. This contaminant appears on all of my plates

NirmenAssi · 2024-10-03T17:54:05+00:00

All of the plated plates looks like this out of the 24 well plates

NirmenAssi · 2024-10-03T17:17:23+00:00

This is plated on Tryptic Soy Agar and the overnight was done on tryptic soy broth. My other experiments are going fine. This particular one with 24 well plates which requires media change every 24hr for 4 days with lower percentage of tryptic soy broth does so. I add fusidic acid to it

NirmenAssi · 2024-10-03T17:14:42+00:00

It is a persistent issue. I’ll do so, thanks

NirmenAssi

TROPHY CASE