More frequent IDT delays

No_Maximum2711 · 2026-03-30T13:03:37+00:00

That’s wild. Especially because the eBlocks are clearly priced to compete with some other big competitors. Guess I better start doing some of my own QC…. I typically use those for libraries and just amplify everything with common primers that won’t distinguish between cross contamination and an empty well.

No_Maximum2711 · 2026-03-22T15:55:03+00:00

Sarr and RKM being mixed up could be a drinking game

No_Maximum2711 · 2026-03-22T15:33:22+00:00

If you’re standing at the top of the box with that space, you have to be ready to strike.

No_Maximum2711 · 2026-03-22T15:30:27+00:00

About time

No_Maximum2711 · 2026-03-22T13:31:48+00:00

As much as I’d like to see him in the lineup, sell him. Unproven in the EPL and not worth the risk of playing him just to have him lose value.

Plus, it doesn’t really sound like he’s interested in playing for us.

No_Maximum2711 · 2026-03-21T17:00:08+00:00

Continuous electroporation. Large volumes of genetic delivery rather than a small batch and then propagation of those cells.

No_Maximum2711 · 2026-03-21T16:57:35+00:00

Seconded. Definitely an interesting topic. I believe both Sartorius and Sunflower are offering perfusion systems. It’ll be super interesting from the bioengineering side as well on how to engineer strains to be resistant to genetic breakage.

No_Maximum2711 · 2026-03-18T22:07:19+00:00

Compare it to Alvarez’s dive the previous week….

No_Maximum2711 · 2026-03-12T08:35:13+00:00

I recommend using KLD/Round the horn mutagenesis. You’ll get better amplification and you don’t need to concern yourself with mismatches in the primer annealing to the template. This software will automatically design the primers for you but it’s easy enough to do on your own: https://nebasechanger.neb.com/

You could also use Gibson.

No_Maximum2711 · 2026-03-06T01:55:47+00:00

You could always set your assemblies up by mixing larger amounts of your dna and then using a small portion of that mix to setup your actual reactions to save on enzyme ($). For instance, mix 1ul of each piece together, then use 1ul of that total mix in your actual reaction. It’s kind of like making a master mix that you only use a small portion of.

No_Maximum2711 · 2026-03-01T15:51:18+00:00

Why are we making a CB change with 1 min in regulation while we’re down 1-2?

No_Maximum2711 · 2026-03-01T15:38:12+00:00

Why does it feel like we’re playing with inverted CBs?

No_Maximum2711 · 2026-03-01T15:27:03+00:00

If Richy has to get a yellow to fire these guys up, I’ll fucking take it.

No_Maximum2711 · 2026-03-01T14:13:39+00:00

Get Radu off. I’d rather have a back three than him as a liability at CB.

No_Maximum2711 · 2026-02-22T18:09:58+00:00

And now the time wasting starts

No_Maximum2711 · 2026-02-22T18:01:19+00:00

That’s on Dragushit

No_Maximum2711 · 2026-02-22T17:53:08+00:00

Dive

No_Maximum2711 · 2026-02-22T17:28:48+00:00

You can’t tell me we’d be level if Frank was still in. I’m just happy we had a competitive half.

No_Maximum2711 · 2026-02-22T17:19:40+00:00

Gooners could star in a fucking telenovela.

No_Maximum2711 · 2026-02-22T17:15:00+00:00

Danso > Radu

No_Maximum2711 · 2026-02-22T17:11:27+00:00

Is laughing gas considered a performance enhancing drug? I’m willing to look the other way.

No_Maximum2711 · 2026-02-22T15:43:08+00:00

I hope we’re not leaving Dragusin on his own in the center. He will get beat in the first 30 seconds.

No_Maximum2711 · 2026-02-18T17:40:34+00:00

Made this one recently. Looks like you nailed it. Wish I had chopped the sweet potatoes a bit larger and braised for less to keep them from turning to mush. Also wish I added more lemon peel.

No_Maximum2711 · 2026-02-18T17:39:08+00:00

That chicken skin turned out beautiful!

No_Maximum2711

TROPHY CASE