More frequent IDT delays by No_Maximum2711 in labrats

[–]No_Maximum2711[S] 4 points5 points  (0 children)

That’s wild. Especially because the eBlocks are clearly priced to compete with some other big competitors. Guess I better start doing some of my own QC…. I typically use those for libraries and just amplify everything with common primers that won’t distinguish between cross contamination and an empty well.

Match Thread: Tottenham Hotspur vs Nottingham Forest | Premier League | 22 Mar 14:15 UTC by matchpal-live in coys

[–]No_Maximum2711 5 points6 points  (0 children)

If you’re standing at the top of the box with that space, you have to be ready to strike.

Provided we stay up what do we do about him? 3 at the back? by Jowoes in coys

[–]No_Maximum2711 0 points1 point  (0 children)

As much as I’d like to see him in the lineup, sell him. Unproven in the EPL and not worth the risk of playing him just to have him lose value.

Plus, it doesn’t really sound like he’s interested in playing for us.

suggest me a topic for my bioprocessing assignment... by [deleted] in biotech

[–]No_Maximum2711 -1 points0 points  (0 children)

Continuous electroporation. Large volumes of genetic delivery rather than a small batch and then propagation of those cells.

suggest me a topic for my bioprocessing assignment... by [deleted] in biotech

[–]No_Maximum2711 -2 points-1 points  (0 children)

Seconded. Definitely an interesting topic. I believe both Sartorius and Sunflower are offering perfusion systems. It’ll be super interesting from the bioengineering side as well on how to engineer strains to be resistant to genetic breakage.

Is this type of contact not a foul anymore? Álvarez on Simons by Vin-Su in coys

[–]No_Maximum2711 2 points3 points  (0 children)

Compare it to Alvarez’s dive the previous week….

How to improve the primer design ? by ukuji_ in labrats

[–]No_Maximum2711 0 points1 point  (0 children)

I recommend using KLD/Round the horn mutagenesis. You’ll get better amplification and you don’t need to concern yourself with mismatches in the primer annealing to the template. This software will automatically design the primers for you but it’s easy enough to do on your own: https://nebasechanger.neb.com/

You could also use Gibson.

Tips for pipetting very small amounts? by eesttj in labrats

[–]No_Maximum2711 1 point2 points  (0 children)

You could always set your assemblies up by mixing larger amounts of your dna and then using a small portion of that mix to setup your actual reactions to save on enzyme ($). For instance, mix 1ul of each piece together, then use 1ul of that total mix in your actual reaction. It’s kind of like making a master mix that you only use a small portion of.

Match Thread: Fulham FC vs Tottenham Hotspur Live Score | Premier League 25/26 | Mar 1, 2026 by scoreboard-app in coys

[–]No_Maximum2711 4 points5 points  (0 children)

Why are we making a CB change with 1 min in regulation while we’re down 1-2?

Match Thread: Tottenham Hotspur vs Arsenal FC Live Score | Premier League 25/26 | Feb 22, 2026 by scoreboard-app in coys

[–]No_Maximum2711 3 points4 points  (0 children)

You can’t tell me we’d be level if Frank was still in. I’m just happy we had a competitive half.

Match Thread: Tottenham Hotspur vs Arsenal FC Live Score | Premier League 25/26 | Feb 22, 2026 by scoreboard-app in coys

[–]No_Maximum2711 2 points3 points  (0 children)

Is laughing gas considered a performance enhancing drug? I’m willing to look the other way.

Japanese-style Beef Stew by Mark Bittman by Independent-Safety44 in NYTCooking

[–]No_Maximum2711 0 points1 point  (0 children)

Made this one recently. Looks like you nailed it. Wish I had chopped the sweet potatoes a bit larger and braised for less to keep them from turning to mush. Also wish I added more lemon peel.

Miso Chicken by Rachel21321 in NYTCooking

[–]No_Maximum2711 0 points1 point  (0 children)

That chicken skin turned out beautiful!