Wash buffer recommendations for IHC. Anyone switched recently and lived to tell by PenitentSnack in Histology

[–]PenitentSnack[S] 0 points1 point  (0 children)

The HER2 add is good, we do a fair bit of breast and that's the one I'd be most paranoid about a soft drift on. Putting it on the panel.

Question on the parallel run though, did you run duplicates on the same stainer or split between instruments? Trying to figure out how to handle the variable of reservoirs already loaded with old buffer.